run_metadata: 9828
This data as json
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 9828 | ERR2102838 | ERX2160149 | ERS1883525 | ERP040145 | PRJEB37796 | Rna seq of control and 2mM NaBu treated zebrafish larvae 8 dpf | E-MTAB-5992 | Transcriptome Analysis | We wanted to compare gene expression from control untreated zebrafish larvae and 2 mM NaBu treated larvae for 24 hours in order to assess the effect of inhibition of the HDAC pathway in these animals | ENA FIRST PUBLIC:2017 08 29|ENA LAST UPDATE:2017 08 23|ArrayExpress:E MTAB 5992 | Protocols: zebrafish were isolated during pharyngula stage daily fed and water changed until 8 dpf 2 mM NaBu for 24 hours in some samples Whole larvae where immersed in RNA later and RNA was extracted using a Qiagen kit following manufacturer recommendations. RNA seq libraries were built using the TruSeq RNA Library Preparation Kit v2 from Illumina RS 122 2001 | C3 control RNA | SAMEA104224507 | Fundacao Champalimaud | ENA first public:2017 08 29|ENA last update:2017 08 23|External Id:SAMEA104224507|INSDC center alias:Fundacao Champalimaud|INSDC center name:Fundacao Champalimaud|INSDC first public:2017 08 29T17:02:02Z|INSDC last update:2017 08 23T09:55:35Z|INSDC status:public|Submitter Id:E MTAB 5992:C3 control RNA|age:8|broker name:ArrayExpress|common name:zebrafish|disease:normal|genotype:wild type genotype|sample name:E MTAB 5992:C3 control RNA|strain:AB | Illumina Genome Analyzer IIx sequencing; Rna seq of control and 2mM NaBu treated zebrafish larvae 8 dpf | E MTAB 5992:C3 control RNA s | C3 control RNA s | Rna seq of control and 2mM NaBu treated zebrafish larvae 8 dpf | zebrafish were isolated during pharyngula stage daily fed and water changed until 8 dpf 2 mM NaBu for 24 hours in some samples Whole larvae where immersed in RNA later and RNA was extracted using a Qiagen kit following manufacturer recommendations. RNA seq libraries were built using the TruSeq RNA Library Preparation Kit v2 from Illumina RS 122 2001 | Experimental Factor: compound:PBS | RNA-Seq | TRANSCRIPTOMIC | RANDOM | SINGLE | ILLUMINA | Illumina Genome Analyzer IIx | ERP040145 | Illumina Genome Analyzer IIx sequencing; Rna seq of control and 2mM NaBu treated zebrafish larvae 8 dpf | ENA FIRST PUBLIC:2017 08 29|ENA LAST UPDATE:2018 11 16 | C3_R1_all.fastq.gz | fastq | 1380662008.0 | 18279108.0 | E MTAB 5992:C3 control RNA | 0:75.53 1:0 | A:345997031;C:338444594;G:316575258;T:379195977;N:449148 | 75 | 0 | 345997031 | 338444594 | 316575258 | 379195977 | 449148 | ERX2160149 | ERS1883525 | ERA1011308 | Fundacao Champalimaud|European Nucleotide Archive | Fundacao Champalimaud|European Nucleotide Archive | 1 | 0.95615 | 0.08131 | 0.68694 | 0.45267 | 74 | B | usable mapping rate | illumina | early_illumina | unknown | random_priming | trueseq | bulk | unknown | unknown | Portugal | 2017-08-23 | Multi-stage | Multi-stage | Whole Organism | All anatomical structures |