run_metadata: 9363
This data as json
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| 9363 | ERR3011945 | ERX3014405 | ERS2994079 | ERP112907 | PRJEB30451 | Effects of anti androgenic compounds on zebrafish insulin mutants | E-MTAB-7283 | Transcriptome Analysis | Aiming to identify insulin independent modulators of glucose homeostasis we performed a drug screen on zebrafish insulin ins mutants and identified androgen receptor AR antagonists. To investigate how AR antagonism mediates glucose level reduction in ins mutants we evaluated the effects of antagonist treatment using transcriptomic studies. RNA Seq analyses were performed on 120 hpf ins mutants treated with Flutamide or Cyproterone starting at 84 hpf compared to vehicle DMSO treated mutants. | ENA FIRST PUBLIC:2018 12 21|ENA LAST UPDATE:2018 12 18 | Protocols: insulin mutant embryos were obtained from two different crosses biological replicates. Embryos were grown at 28 degrees C in egg water. 10 animals per sample were pooled water was removed and Trizol was added. Animals were treated with 1% DMSO or Flutamide 10 micromolar or Cyproterone 10 micromolar from 84 hpf to 120 hpf. Total RNA was isolated from 120 hpf zebrafish using the RNA Clean & Concentrator kit Zymo Research combined with DNase digestion RNase free DNase Set Promega to avoid contamination by genomic DNA. 3µg of total RNA was used as input for Truseq Stranded mRNA Library preparation following manufacture's low sample protocol Illumina | DMSO 2 | SAMEA5186580 | Max Planck Institute for Heart and Lung Research | ENA FIRST PUBLIC:2018 12 21T17:03:09Z|ENA LAST UPDATE:2018 12 18T09:28:56Z|External Id:SAMEA5186580|INSDC center name:Max Planck Institute for Heart and Lung Research|INSDC first public:2018 12 21T17:03:09Z|INSDC last update:2018 12 18T09:28:56Z|INSDC status:public|Submitter Id:E MTAB 7283:DMSO 2|age:120|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:ins CRISPR/Cas9 mediated knockout|individual:mixed pool of 10 embryos|organism part:whole organism|sample name:E MTAB 7283:DMSO 2|scientific name:Danio rerio|strain:ins bns102 | NextSeq 500 sequencing; Effects of anti androgenic compounds on zebrafish insulin mutants | E MTAB 7283:DMSO 2 s | DMSO 2 s | Effects of anti androgenic compounds on zebrafish insulin mutants | insulin mutant embryos were obtained from two different crosses biological replicates. Embryos were grown at 28 degrees C in egg water. 10 animals per sample were pooled water was removed and Trizol was added. Animals were treated with 1% DMSO or Flutamide 10 micromolar or Cyproterone 10 micromolar from 84 hpf to 120 hpf. Total RNA was isolated from 120 hpf zebrafish using the RNA Clean & Concentrator kit Zymo Research combined with DNase digestion RNase free DNase Set Promega to avoid contamination by genomic DNA. 3µg of total RNA was used as input for Truseq Stranded mRNA Library preparation following manufacture's low sample protocol Illumina | Experimental Factor: compound:dimethyl sulfoxide|Experimental Factor: dose:1 | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | NextSeq 500 | ERP112907 | NextSeq 500 sequencing; Effects of anti androgenic compounds on zebrafish insulin mutants | ENA FIRST PUBLIC:2018 12 21|ENA LAST UPDATE:2018 12 18 | Teja_DMSO_2_R1.fastq.gz | fastq | 1811573293.0 | 24316929.0 | E MTAB 7283:DMSO 2 | 0:74.50 1:0 | A:470888315;C:423635861;G:396796840;T:520242179;N:10098 | 74 | 0 | 470888315 | 423635861 | 396796840 | 520242179 | 10098 | ERX3014405 | ERS2994079 | ERA1697296 | European Nucleotide Archive | European Nucleotide Archive | 1 | 0.94219 | 0.12305 | 0.67184 | 0.47704 | 75 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Unknown | 2018-12-18 | Larval | Larval | Embryo Imprecise | All anatomical structures |