run_metadata: 77322
This data as json
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| 77322 | SRR25453697 | SRX21186613 | SRS18446634 | SRP452067 | PRJNA999747 | Transcriptomic differences between juvenile and adult quiescent NSCs | GSE239597 | Transcriptome Analysis | We generated a dataset in which we compared the bulk transcriptome of qNSCs between 1.5 and 3.5 mpf fish stages between which deeper quiescence is progressively instated in order to find differences between deeply and shallow quiescent NSC Overall design: To investigate transcriptomic differences between adult deeply quiescent NSCs and activated NSCs we performed bulk RNA Seq | parent bioproject:PRJNA999741 | pubmed:38518783 | Experiment 3 tgher4:RFP | GSM7668487 | source name:brain/telencephali|tissue:brain/telencephali|genotype:tgher4:RFP|nsc status:Quiescent|cell type:R|region:tel|age:15m|replicate:r3|geo loc name:missing|collection date:missing | Experiment 3 tgher4:RFP | Reads were mapped to zebrafish genome using STAR Gene counts were computed using featureCounts Differential expression analysis was performed with DESeq2 Assembly: GRCz11 Supplementary files format and content: Tab delimited text file generated by featureCounts | brain/telencephali | PicoPure Isolation kit Life technologies RNase free DNase Set Qiagen® SMARTer® Stranded Total RNA Seq Kit Pico Input Mammalian kit Clontech Laboratories | tissue:brain/telencephali|genotype:tgher4:RFP|nsc status:Quiescent|cell type:R|region:tel|age:15m|replicate:r3 | GSM7668487 | GSM7668487: Experiment 3 tgher4:RFP; Danio rerio; RNA Seq | GSM7668487 r1 | GSM7668487 | 1 | PicoPure Isolation kit Life technologies RNase free DNase Set Qiagen® SMARTer® Stranded Total RNA Seq Kit Pico Input Mammalian kit Clontech Laboratories | RNA-Seq | TRANSCRIPTOMIC | cDNA | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP452067 | 3R_GAATTCGT_L007_R1_001.fastq.gz | fastq | 1536622230.0 | 23640342.0 | GSM7668487 r1 | 0:65 | A:400302281;C:383386741;G:418378970;T:334487419;N:66819 | 65 | 400302281 | 383386741 | 418378970 | 334487419 | 66819 | SRX21186613 | SRS18446634 | SRA1682605 | Institut Pasteur | Institut Pasteur | 1 | 0.87737 | 0.36524 | 0.72991 | 0.5329 | 65 | B | usable mapping rate | illumina | hiseq_era | full_length | cdna_unspecified | smarter | bulk | bulk | bulk | France | 2023-07-28 | Undetermined | Multi-stage | Brain | Nervous System |