run_metadata: 77243
This data as json
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| 77243 | SRR25536619 | SRX21265875 | SRS18518718 | SRP451621 | PRJNA998935 | A new cholesterol diet protocol for zebrafish reveals hypercholesterolemia and fasting associated hepatic steatosis | PRJNA998935 | Other | Zebrafish are an ideal model organism to study lipid metabolism and to elucidate the molecular underpinnings of human lipid associated disorders. In this study we provide a protocol to assay the impact of a high cholesterol diet HCD on lipid deposition and lipoprotein regulation. Fish fed HCD developed hypercholesterolemia as indicated by significantly elevated ApoB containing lipoproteins ApoB LP and increased plasma levels of cholesterol and cholesterol esters CE. Feeding of the HCD was also associated with a fatty liver phenotype presented as severe hepatic steatosis in zebrafish larvae post feeding the diet for 8 days followed by fasting for 24 hours. Additionally 30 percent of all adult female fish were observed to develop hepatic steatosis post feeding HCD for 2 weeks followed by 3 days of fasting. The HCD feeding paradigm produced elevated levels of liver triacylglycerol TG free cholesterol and CE compared to fish fed the control diet. The doubling of liver TG was striking because our HCD was only supplemented with cholesterol. The accumulated TG in the liver was not likely due to increase de novo lipogenesis or inhibited beta oxidation since we identified no differentially expressed genes in these pathways between the livers of fish fed the HCD and the control diets. However fasted HCD fish had significantly increased expression of the lipogenesis gene fasn in adipose tissue and higher free fatty acids FFA in plasma. This suggested that elevated dietary cholesterol resulted in lipid accumulation in adipocytes which supplied more FFA during fasting promoting hepatic steatosis. In conclusion our HCD zebrafish protocol represents an effective and reliable approach for studying the temporal characteristics of the physiological and biochemical responses to high levels of dietary cholesterol and provides insights into the mechanisms that may underlie fatty liver disease. | C m LV 3 | breed:AB|dev stage:Adult|collection date:2021 12 01|geo loc name:USA: Baltimore|sex:male|tissue:Liver|treatment:Control Diet|Replicate:Biological replicate 3|BioSampleModel:Model organism or animal | RNAseq of zebrafish liver | C m 20 | C m 20 | Liver from fish fed either control or high cholesterol diets | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiSeq | SRP451621 | Control_Liver_Male_Fish20_S1_L001_R1_001.fastq.gz Control_Liver_Male_Fish20_S1_L002_R1_001.fastq.gz Control_Liver_Male_Fish20_S1_L003_R1_001.fastq.gz Control_Liver_Male_Fish20_S1_L004_R1_001.fastq.gz | fastq fastq fastq fastq | 3516477150.0 | 46886362.0 | Control Liver Male Fish20 S1 L001 R1 001.fastq.gz | 0:75 | A:881536366;C:786657878;G:831133599;T:1017082181;N:67126 | 75 | 881536366 | 786657878 | 831133599 | 1017082181 | 67126 | SRX21265875 | SRS18518718 | SRA1687253 | Carnegie Insitution for Science|Embryology | Carnegie Insitution for Science | 1 | 0.93731 | 0.06298 | 0.81257 | 0.55948 | 75 | B | usable mapping rate | illumina | miseq | unknown | poly_a | unknown | bulk | unknown | unknown | United States | 2023-08-06 | Adult | Adult | Liver | Liver and Biliary System |