run_metadata: 75600
This data as json
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 75600 | SRR24709480 | SRX20488148 | SRS17798895 | SRP439167 | PRJNA975174 | Bulk RNA seq investigating differential gene regulation across gata3 knockdown and rescue | PRJNA975174 | Other | Loss of the transcription factor Gata3 associates with the highly variable human birth defects HDR syndrome and microsomia and can lead to disruption of the neural crest derived facial skeleton. RNA was isolated from cranial neural crest cells of zebrafish embryos at 28 hpf in control gata3 knockdown and gata3 overexpression embryos. Goal of the study was to illustrate multiple potential pathways for Gata3 function and demonstrate a systematic unbiased process to identify modifiers of genotype phenotype correlations. | gata3 MO injected fish gata3 overexpression replicate 2 | Gata3 rescue embryos replicate 2 | HS2 | strain:Not collected|isolate:Not collected|breed:Not collected|cultivar:Not collected|ecotype:Not collected|age:Not determined|dev stage:28 hpf|sex:Not determined|tissue:Cranial neural crest cells|treatment:Gata3 Mo OE|BioSampleModel:Model organism or animal | Gata3 rescue embryos replicate 2 | HS2 | HS2 | 28 hpf zebrafish CNCC gata3 MO gata3 OE rep 2 | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP439167 | hs-2_S43_L004_R1_001.fastq.gz hs-2_S43_L004_R2_001.fastq.gz | fastq fastq | 18888379506.0 | 62544303.0 | hs 2 S43 L004 R1 001.fastq.gz | 0:151 1:151 | A:5096840826;C:4346330631;G:4477598678;T:4966787816;N:821555 | 151 | 151 | 5096840826 | 4346330631 | 4477598678 | 4966787816 | 821555 | SRX20488148 | SRS17798895 | SRA1643035 | University of Texas at Austin|Department of Molecular Biosciences | University of Texas at Austin | 2 | 0.94359 | 0.95104 | 0.10627 | 0.10498 | 0.69775 | 0.69645 | 0.46042 | 0.46973 | 151 | 151 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | cdna_unspecified | unknown | bulk | bulk | bulk | United States | 2023-05-23 | Pharyngula | Embryo | Brain | Nervous System |