run_metadata: 74702

This data as json

rowid	run.accession	experiment.accession	sample.accession	study.accession	bioproject	study.title	study.alias	study.type	study.abstract	study.attributes	study.PMIDs	sample.description	sample.title	sample.alias	sample.centername	sample.attributes	GEOsample.title	GEOsample.dataprocessing	GEOsample.source	GEOsample.treatmentprotocol	GEOsample.extractprotocol	GEOsample.growthprotocol	GEOsample.characteristics	GEOsample.accession	experiment.title	experiment.alias	experiment.library_name	experiment.design_description	experiment.library_construction_protocol	experiment.attributes	experiment.library_strategy	experiment.library_source	experiment.library_selection	experiment.library_layout	experiment.platform	experiment.instrument_model	experiment.spot_descriptor	experiment.study_ref	run.title	run.attributes	run.filename	run.semantic_name	run.total_bases	run.total_spots	run.alias	run.read_lengths	run.base_counts	run.r1_length	run.r2_length	run.r3_length	run.r4_length	run.Acount	run.Ccount	run.Gcount	run.Tcount	run.Ncount	run.experiment	run.pool_member	submission.accession	submission.srasource	submission.bioprojectsource	seqdetective.n_mates	seqdetective.mapping_rate.mate1	seqdetective.mapping_rate.mate2	seqdetective.nofeature_rate.mate1	seqdetective.nofeature_rate.mate2	seqdetective.sparsity.mate1	seqdetective.sparsity.mate2	seqdetective.pos_strand_rate.mate1	seqdetective.pos_strand_rate.mate2	seqdetective.readlen.mate1	seqdetective.readlen.mate2	seqdetective.judgement.mate1	seqdetective.judgement.mate2	seqdetective.judgement.reason	platform_family	instrument_generation	read_bias	selection_class	prep_kit	sc_or_bulk	tech_class	technology	tech_variant	submission.bioprojectsource.country	earliest_date	devstage_curation	devstage_curation_coarse	tissue_curation	tissue_curation_coarse
74702	SRR24003727	SRX19807141	SRS17168877	SRP429947	PRJNA949785	Sequencing of zebrafish bone tissues	PRJNA949785	Other	Transcriptomics was used to investigate sensitive genes associated with exercise improved bone mass					eight month zebrafish bone tissues		breed:Not applicable\|age:8 month\|sex:male\|tissue:b1\|BioSampleModel:Model organism or animal									RNA Seq of b1 tissue in zebrafish	case2 3	case2 3	Qualified total RNA was purified by DNase I and mRNA was enriched by adding OligodT attached magnetic beads. Subsequently fragment buffer was added to cleave the mRNA into small fragments. Fragmented mRNA was used as a template for reverse transcription to generate the first cDNA strand and then the second cDNA strand was generated. End repair and adaptor ligation were performed by adding A tailing Mix and RNA Index Adapters and then PCR amplification was performed. Single strand circle DNA library was obtained by recovering and cycling the PCR products. The final library was obtained by purifying the linear DNA molecules that were not cycled. The quality of the final library was tested. The qualified library was replicated by rolling circle replication to form DNA nanospheres DNBs. DNBs were added to the mesh holes of a nanochip using high density DNA nanochip technology. Subsequently sequencing was performed on the BGIISEQ500 platform BGI Shenzhen Guangdong China using combined probe anchored polymerisation.			RNA-Seq	TRANSCRIPTOMIC	PCR	PAIRED	BGISEQ	BGISEQ-500		SRP429947			case2_3_1.fq.gz case2_3_2.fq.gz	fastq fastq	6706743300.0	22355811.0	case2 3 1.fq.gz	0:150 1:150	A:1845685627;C:1507725453;G:1499541624;T:1853790596;N:0	150	150			1845685627	1507725453	1499541624	1853790596	0	SRX19807141	SRS17168877	SRA1612461	Sun Yat-sen University\|Department of Epidemiology, School of Public Healt	Sun Yat-sen University	2	0.94268	0.93594	0.08009	0.07937	0.70402	0.70441	0.4556	0.48818	150	150	B	B	biological fallback assumption	bgi	bgi	unknown	random_priming	unknown	bulk	unknown	unknown		China	2023-03-29	Adult	Adult	Bone or Cartilage	Skeletal Element