run_metadata: 74566

This data as json

rowid	run.accession	experiment.accession	sample.accession	study.accession	bioproject	study.title	study.alias	study.type	study.abstract	study.attributes	study.PMIDs	sample.description	sample.title	sample.alias	sample.centername	sample.attributes	GEOsample.title	GEOsample.dataprocessing	GEOsample.source	GEOsample.treatmentprotocol	GEOsample.extractprotocol	GEOsample.growthprotocol	GEOsample.characteristics	GEOsample.accession	experiment.title	experiment.alias	experiment.library_name	experiment.design_description	experiment.library_construction_protocol	experiment.attributes	experiment.library_strategy	experiment.library_source	experiment.library_selection	experiment.library_layout	experiment.platform	experiment.instrument_model	experiment.spot_descriptor	experiment.study_ref	run.title	run.attributes	run.filename	run.semantic_name	run.total_bases	run.total_spots	run.alias	run.read_lengths	run.base_counts	run.r1_length	run.r2_length	run.r3_length	run.r4_length	run.Acount	run.Ccount	run.Gcount	run.Tcount	run.Ncount	run.experiment	run.pool_member	submission.accession	submission.srasource	submission.bioprojectsource	seqdetective.n_mates	seqdetective.mapping_rate.mate1	seqdetective.mapping_rate.mate2	seqdetective.nofeature_rate.mate1	seqdetective.nofeature_rate.mate2	seqdetective.sparsity.mate1	seqdetective.sparsity.mate2	seqdetective.pos_strand_rate.mate1	seqdetective.pos_strand_rate.mate2	seqdetective.readlen.mate1	seqdetective.readlen.mate2	seqdetective.judgement.mate1	seqdetective.judgement.mate2	seqdetective.judgement.reason	platform_family	instrument_generation	read_bias	selection_class	prep_kit	sc_or_bulk	tech_class	technology	tech_variant	submission.bioprojectsource.country	earliest_date	devstage_curation	devstage_curation_coarse	tissue_curation	tissue_curation_coarse
74566	SRR23829124	SRX19650812	SRS17019464	SRP426991	PRJNA944150	Absence of telomerase restricts melanoma development.	PRJNA944150	Other	Most cancers reactivate telomerase to maintain telomere length to acquire immortality. The importance of this process is well illustrated by the fact that telomerase promoter mutations are found at a high frequency in many cancer types including melanoma. However it is unclear when and if telomerase is strictly required during tumorigenesis. Here we show that melanoma can occur in the absence of telomerase. Nevertheless it is required to sustain later growth and avoid regression. Using telomerase mutant zebrafish tert / combined with two established melanoma models we found equal melanoma incidence and invasiveness as tumors became visible. Later however while WT fish develop increasing larger tumors tert / tumors stagnate growth and regress. Consistently tert / tumors showed lower cell proliferation higher apoptosis and melanocyte differentiation. We also detected an immune response to tert / tumors. Indeed tert / tumors resumed growth when transplanted to immunocompromised hosts. We propose that telomerase is required for melanoma in zebrafish albeit at later stages of progression to sustain growth while avoiding immune rejection and regression. Thus absence of telomerase restricts melanoma through tumor autonomous mechanisms cell cycle arrest apoptosis and melanocyte differentiation and a non tumor autonomous mechanisms immune rejection.				3006/7	4B		strain:AB\|age:3 month\|sex:male\|tissue:Tumor\|birth date:10.03.2021\|birth location:PEMAV IRCAN France\|death date:30.06.2021\|genotype:tert+/+ rep1\|BioSampleModel:Model organism or animal									RNA Seq of Danio rerio : 3months male tumor	4B	4B	RNA Seq of Danio rerio : 3months male tumor			RNA-Seq	TRANSCRIPTOMIC	Oligo-dT	PAIRED	BGISEQ	BGISEQ-500		SRP426991			4B_1.fq.gz 4B_2.fq.gz	fastq fastq	10792140600.0	53960703.0	4B 1.fq.gz	0:100 1:100	A:2882693797;C:2464129073;G:2535344124;T:2909973606;N:0	100	100			2882693797	2464129073	2535344124	2909973606	0	SRX19650812	SRS17019464	SRA1603485	Sun Yat-sen University Cancer Center\|State Key Laboratory of Oncology in South China	Sun Yat-sen University Cancer Center Miguel Godinho Ferreira	2	0.91884	0.92345	0.12967	0.12884	0.74117	0.74103	0.50792	0.51065	100	100	B	B	biological fallback assumption	bgi	bgi	unknown	poly_a	unknown	bulk	unknown	unknown		China	2023-03-14	Adult	Adult	Cancer or Tumor	Cancer or Tumor