run_metadata: 69480
This data as json
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| 69480 | SRR18731941 | SRX14832178 | SRS12587950 | SRP369810 | PRJNA825753 | Zebrafish ovarian transcriptome sequencing | PRJNA825753 | Other | 1 The transcriptome sequencing of nine samples was completed and a total of 63.66 Gb Clean Data was obtained and the Clean Data of each sample reached 6.14 Gb and above and the percentage of Q30 bases was 91.64 % and above.2 The Clean Reads of each sample were compared with the designated reference genome for sequence alignment and the alignment efficiency was 92.04% or more.3 Functional annotation was performed for all genes including comparison with NR [1] Swiss Prot [2] KEGG [3] COG [4] KOG [5] GO [6] and Pfam databases and annotation results for a total of 26929 genes were obtained.4 Based on the comparison results the libraries were evaluated.5 Based on the comparison results gene expression analysis was performed. Based on the expression of genes in different samples differentially expressed genes were identified and functional annotation and enrichment analysis were performed. | Zebrafish Ovaries | strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:6 Months|dev stage:adult fish|sex:female|tissue:Ovaries|BioSampleModel:Model organism or animal | Adult zebrafish ovary | 20220412 8 | 20220412 8 | materials and methods | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2500 | SRP369810 | GPR19-11-Rescue-2_BKDL202619368-1a-N710-AK416_1.fq.gz GPR19-11-Rescue-2_BKDL202619368-1a-N710-AK416_2.fq.gz | fastq fastq | 6295515300.0 | 20985051.0 | GPR19 11 Rescue 2 BKDL202619368 1a N710 AK416 1.fq.gz | 0:150 1:150 | A:1677101982;C:1466375952;G:1498052845;T:1653959638;N:24883 | 150 | 150 | 1677101982 | 1466375952 | 1498052845 | 1653959638 | 24883 | SRX14832178 | SRS12587950 | SRA1402797 | zhejiang ocean university|school of fishery | zhejiang ocean university | 2 | 0.94529 | 0.94401 | 0.0307 | 0.03031 | 0.73963 | 0.74126 | 0.49539 | 0.49777 | 150 | 150 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2022-04-12 | Adult | Adult | Gonad | Reproductive System |