run_metadata: 68358

This data as json

rowid	run.accession	experiment.accession	sample.accession	study.accession	bioproject	study.title	study.alias	study.type	study.abstract	study.attributes	study.PMIDs	sample.description	sample.title	sample.alias	sample.centername	sample.attributes	GEOsample.title	GEOsample.dataprocessing	GEOsample.source	GEOsample.treatmentprotocol	GEOsample.extractprotocol	GEOsample.growthprotocol	GEOsample.characteristics	GEOsample.accession	experiment.title	experiment.alias	experiment.library_name	experiment.design_description	experiment.library_construction_protocol	experiment.attributes	experiment.library_strategy	experiment.library_source	experiment.library_selection	experiment.library_layout	experiment.platform	experiment.instrument_model	experiment.spot_descriptor	experiment.study_ref	run.title	run.attributes	run.filename	run.semantic_name	run.total_bases	run.total_spots	run.alias	run.read_lengths	run.base_counts	run.r1_length	run.r2_length	run.r3_length	run.r4_length	run.Acount	run.Ccount	run.Gcount	run.Tcount	run.Ncount	run.experiment	run.pool_member	submission.accession	submission.srasource	submission.bioprojectsource	seqdetective.n_mates	seqdetective.mapping_rate.mate1	seqdetective.mapping_rate.mate2	seqdetective.nofeature_rate.mate1	seqdetective.nofeature_rate.mate2	seqdetective.sparsity.mate1	seqdetective.sparsity.mate2	seqdetective.pos_strand_rate.mate1	seqdetective.pos_strand_rate.mate2	seqdetective.readlen.mate1	seqdetective.readlen.mate2	seqdetective.judgement.mate1	seqdetective.judgement.mate2	seqdetective.judgement.reason	platform_family	instrument_generation	read_bias	selection_class	prep_kit	sc_or_bulk	tech_class	technology	tech_variant	submission.bioprojectsource.country	earliest_date	devstage_curation	devstage_curation_coarse	tissue_curation	tissue_curation_coarse
68358	SRR17714701	SRX13877708	SRS11747880	SRP356388	PRJNA799842	RNA seq of CRISPR/Cas9 mediated genome editing zebrafishes; the alligator pipefish spleen and the small white organ of the lined seahorse.	PRJNA799842	Other	To explore the function of the gene in spleen development we generated a knockout zebrafish line. To investigate whether the phenotypic consequences in the seahorses were caused by a missense mutation in this gene we generated a point mutation zebrafish line by CRISPR/Cas9 mediated homologous recombination HR. RNA seq of these samples tissues brain liver kidney and intestine were analyzed. In addition to clarify the splenic phenotype of the Syngnathidaes the transcriptomic profiles of the S. biaculeatus spleens and the H. erectus small white organ were also sampled and sequenced.					MU ki 4		strain:AB type\|isolate:point mutation\|breed:MU ki 4\|age:3 month\|dev stage:NA\|sex:NA\|tissue:kidney\|BioSampleModel:Model organism or animal									RNA seq of zebrafish	MU ki 4	MU ki 4	RNA seq			RNA-Seq	TRANSCRIPTOMIC	PolyA	PAIRED	ILLUMINA	Illumina HiSeq 2500		SRP356388		loader:fastq load.py	Daniorerio_MU-ki-4_good_1.fq.gz Daniorerio_MU-ki-4_good_2.fq.gz	fastq fastq	6642468464.0	22227277.0	Daniorerio MU ki 4 good 1.fq.gz	0:149.42 1:149.42	A:1806510861;C:1499915463;G:1544497199;T:1791210925;N:334016	149	149			1806510861	1499915463	1544497199	1791210925	334016	SRX13877708	SRS11747880	SRA1360632	South China Sea Institute of Oceanology\|CAS Key Laboratory of Tropical Marine Bio-Resource	South China Sea Institute of Oceanology	2	0.93502	0.93305	0.0993	0.09919	0.68519	0.68617	0.50471	0.502	150	150	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown		China	2022-01-27	Adult	Adult	Kidney	Renal System