run_metadata: 67690
This data as json
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| 67690 | SRR17235500 | SRX13414604 | SRS11316485 | SRP350846 | PRJNA789116 | RNAseq of Bbs1 KO Zebrafish larval eyes | PRJNA789116 | Other | Bardet Biedl syndrome BBS is a ciliopathy characterized by retinal dystrophy intellectual disability polydactyly obesity and renal anomalies. In photoreceptors PR light sensation occurs in outer segments OSs which are specialized primary cilia. BBS1 the major BBS gene is part of a protein complex called BBSome which is involved in intracellular protein transport. However the precise function of BBS1/BBSome in controlling ciliary content in PRs remains unclear. To investigate the transcriptional consequences of a dysfunctional BBSome we established a new bbs1 zebrafish mutant. We conducted an eye specific RNAseq experiment using 5 dpf and 10 dpf maternal zygotic mutants and their heterozygous unaffected siblings as control. Differential expression analysis was used to investigate the expression levels at 5 and 10 days. | BBS1 CTRL 5dpf S3 | strain:Bbs1ka742|age:5dpf|dev stage:Larvae|sex:not applicable|tissue:Eye|genotype:+/ |Paired group:C|BioSampleModel:Model organism or animal | RNA Seq of Danio Rerio: larval bbs1 mutant eye | BBS1 CTRL 5dpf S3 | BBS1 CTRL 5dpf S3 | TruSeq Stranded Total RNA Library Prep Gold | RNA-Seq | TRANSCRIPTOMIC | unspecified | SINGLE | ILLUMINA | Illumina NovaSeq 6000 | SRP350846 | fasta file:Danio rerio.GRCz11.dna.primary assembly.fa|assembly:GCA 000002035.4 | BBS1_CTRL_5dpf_S3_merged.bam Danio_rerio.GRCz11.dna.primary_assembly.fa | bam bam | 3505192259.0 | 35384389.0 | BBS1 CTRL 5dpf S3 merged.bam | 0:99.06 | A:910433958;C:775887812;G:822289434;T:996540997;N:40058 | 99 | 910433958 | 775887812 | 822289434 | 996540997 | 40058 | SRX13414604 | SRS11316485 | SRA1343787 | University of Zurich (UZH)|Molecular Life Science | University of Zurich (UZH) | 1 | 0.94274 | 0.28843 | 0.68678 | 0.47383 | 101 | B | usable mapping rate | illumina | novaseq_era | unknown | unknown | trueseq | bulk | bulk | bulk | Switzerland | 2021-12-15 | Larval | Larval | Eye | Sensory System |