run_metadata: 66974
This data as json
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| 66974 | SRR16959538 | SRX13151074 | SRS11083380 | SRP346418 | PRJNA780940 | transcriptomics of difenoconazole and dimethomorph on zebrafish embryos | PRJNA780940 | Other | Difenoconazole DIF and dimethomorph DIM are widely used pesticides frequently detected together in environmental samples so the deleterious effects of combined exposure warrant detailed examination. In this study the individual and combined effects of DIM and DIF on conventional developmental parameters hatch rate deformity rate lethality and gene expression were measured in embryonic zebrafish. Both DIF and DIM interfered with normal zebrafish embryo development and the most sensitive toxicity index for both was 96 hpf deformity rate BMDL10 values of 0.30 and 1.10 mg/L respectively. The combination of DIF and DIM had mainly synergistic deleterious effects on 96 hpf deformity and mortality rates. Transcriptome analysis showed that these compounds markedly downregulated expression of mcm family genes cdk1 and cdc20 thereby potentially disrupting DNA replication and cell cycle progression. Enhanced surveillance for this pesticide combination is recommended as simultaneous environmental exposure may be substantially more harmful than exposure to either compound alone. | 1+mix | strain:Tu|isolate:N/A|breed:N/A|cultivar:N/A|ecotype:N/A|age:10 96h|dev stage:larvae|sex:male and female|tissue:larvae|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio : mixture group | mix 1 | mix 1 | The final library was amplified with phi29 to make DNA nanoballs DNB with more than 300 copies of one molecular. | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | BGISEQ | BGISEQ-500 | SRP346418 | mix1_3_2.fq.gz mix1_1_1.fq.gz | fastq fastq | 6639699600.0 | 44264664.0 | mix1 1 1.fq.gz | 0:150 1:150 | A:1738687157;C:1575344731;G:1569683716;T:1755974708;N:9288 | 150 | 150 | 1738687157 | 1575344731 | 1569683716 | 1755974708 | 9288 | SRX13151074 | SRS11083380 | SRA1330365 | China Agricultural University|College of animal science and technology | China Agricultural University | 1 | 0.95161 | 0.05697 | 0.6817 | 0.48485 | 150 | B | usable mapping rate | bgi | bgi | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2021-11-16 | Larval | Larval | Undetermined | Undetermined |