run_metadata: 66746
This data as json
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| 66746 | SRR16530664 | SRX12733275 | SRS10681587 | SRP342649 | PRJNA773625 | loft ventricle RNAseq | PRJNA773625 | Other | Cardiac looping and trabeculation are key processes during cardiac chamber maturation and alteration of these processes frequently causes congenital heart defects. Here we report the isolation cloning and characterization of the proprotein convertase furina from the cardiovascular mutant loft in zebrafish and thus establish its critical role in heart looping and trabecular development. Although heart defects are previously reported in zebrafish and mouse furin mutants it remains unclear what are the targets of Furin in the heart. This work presents a new layer of post translational modulation of Notch1b proteins by Furina during zebrafish cardiac looping and trabeculation and mutations in the Furina Notch1b axis may shed light on the genetic basis of congenital heart diseases in humans. | RNAseq of zebrafish heart | loft mutant RNAseq rep1 | strain:Tu|dev stage:3 dpf determined|tissue:ventricle|genotype:loft mutant|replicate:biological replicate 1|BioSampleModel:Model organism or animal | RNAseq of zebrafish heart | loft mutant RNAseq rep1 | loft mutant RNAseq rep1 | The cDNA libraries were constructed by MALBAC method KT110700424 Yikon Genomics | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | PAIRED | ILLUMINA | Illumina HiSeq 2500 | SRP342649 | assembly:danRer7 | loft_mutant_RNAseq_rep1.1.bam loft_mutant_RNAseq_rep1.2.bam | bam bam | 4349194532.0 | 21530666.0 | loft mutant RNAseq rep1.1.bam | 0:101 1:101 | A:1285307324;C:607005074;G:876055199;T:1580773245;N:53690 | 101 | 101 | 1285307324 | 607005074 | 876055199 | 1580773245 | 53690 | SRX12733275 | SRS10681587 | SRA1315131 | Peking University|Institute of Molecular Medicine | Peking University | 2 | 0.50395 | 0.50527 | 0.2163 | 0.21684 | 0.87444 | 0.88371 | 0.47374 | 0.46918 | 101 | 101 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | other | unknown | bulk | unknown | unknown | China | 2021-10-22 | Larval | Larval | Heart | Cardiovascular System |