run_metadata: 66306
This data as json
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 66306 | SRR16292402 | SRX12571554 | SRS10528527 | SRP340859 | PRJNA770361 | Transcriptome analysis of scd5 homologous deficiency zebrafish | PRJNA770361 | Other | Detection of expression changes in zebrafish visceral adipose and subcutaneous adipose tissue transcriptome under homologous deficiency of scd5 and dietary supplement of w3 PUFA. | scd5 VAT | strain:scd5 / |dev stage:4mpf|sex:male|tissue:visceral adipose tissue|BioSampleModel:Model organism or animal | RNA seq of scd5 / Danio rario: adult male VAT | scd5 0 L | scd5 0 L | RNA seq transcriptome librariy was prepared following TruSeqTM RNA sample preparation Kit from Illumina San Diego CA using 5g of total RNA. Shortly messenger RNA was isolated according to polyA selection method by oligodT beads and then fragmented by fragmentation buffer firstly. Secondly double stranded cDNA was synthesized using a SuperScript double stranded cDNA synthesis kit Invitrogen CA with random hexamer primers Illumina. Then the synthesized cDNA was subjected to end repair phosphorylation and A base addition according to Illuminas library construction protocol. Libraries were size selected for cDNA target fragments of 200300 bp on 2% Low Range Ultra Agarose followed by PCR amplified using Phusion DNA polymerase NEB for 15 PCR cycles. post quantified by TBS380 paired end RNA seq sequencing library was sequenced with the Illumina HiSeq 4000 2 150bp read length. | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | PAIRED | ILLUMINA | Illumina HiSeq 4000 | SRP340859 | scd0_L.R1.fastq.gz scd0_L.R2.fastq.gz | fastq fastq | 6940147542.0 | 22980621.0 | scd0 L.R1.fastq.gz | 0:151 1:151 | A:1777349154;C:1663070037;G:1697971696;T:1801650476;N:106179 | 151 | 151 | 1777349154 | 1663070037 | 1697971696 | 1801650476 | 106179 | SRX12571554 | SRS10528527 | Children's Hospital of Fudan University | 2 | 0.94273 | 0.93332 | 0.03159 | 0.03113 | 0.76753 | 0.76887 | 0.56506 | 0.56717 | 151 | 151 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | trueseq | bulk | unknown | unknown | China | 2021-10-11 | Adult | Adult | Adipose Tissue | Adipose Tissue |