run_metadata: 66118
This data as json
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| 66118 | SRR15910173 | SRX12200568 | SRS10176857 | SRP337313 | PRJNA763718 | Management of hypercholesterolemia through dietary beta glucans in a zebrafish model | PRJNA763718 | Other | Consumption of lipid rich and highly processed foods can increase the cholesterol content in the blood leading to unfavourable disease conditions. beta glucans are known to have hypocholesterolemic effect however subtle changes in their molecular branching can influence their bioactivity. Therefore a comparative investigation of the cholesterol lowering potential of dietary beta glucans from oats and a microalga and simvastatin a well known cholesterol lowering drug was undertaken employing the zebrafish model of hypercholesterolemia.We conducted a twelve week long feeding experiment employing adult male zebrafish. The fish were allocated to 5 dietary treatments; a control group a high cholesterol group two beta glucan groups and a simvastatin group.The intestinal transcriptome of the zebrafish responded to dietary cholesterol indicating the possible suppression of endogenous cholesterol biosynthesis pathways dysfunction of both endoplasmic reticulum and mitochondria and brought about histological changes in the intestine. The two beta glucans and simvastatin significantly mitigated the rise in plasma cholesterol levels restored the expression of several genes and possibly alleviated the endoplasmic reticulum related effects induced by dietary cholesterol. Oat and microalgal beta glucan feeding induced distinct patterns of gene expression changes which revealed antagonistic effect of the two beta glucans on fatty acid metabolism protein catabolic processes and nuclear division in the intestine. | Algal glucan | alga 2 | strain:Wild type|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:laboratory|age:70 weeks|dev stage:adult|sex:male|tissue:intestine|collected by:Adnan Hussain Gora|collection date:2020 09|treatment:algalglucanAG replicate2|BioSampleModel:Model organism or animal | RNA Seq of zebrafish | AG2 | AG2 | PCR enrichment of adaptor ligated DNA | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 550 | SRP337313 | alga_2_S11_R1_001.fastq.gz | fastq | 2667876981.0 | 35347747.0 | alga 2 S11 R1 001.fastq.gz | 0:75.48 1:0 | A:608681566;C:714247949;G:658536241;T:684669115;N:1742110 | 75 | 0 | 608681566 | 714247949 | 658536241 | 684669115 | 1742110 | SRX12200568 | SRS10176857 | SRA1295228 | Nord University|Faculty of Biosciences and Aquaculture | Nord University | 1 | 0.9577 | 0.13465 | 0.77425 | 0.60177 | 76 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | unknown | bulk | unknown | unknown | Norway | 2021-09-16 | Adult | Adult | Gut | Digestive System |