run_metadata: 66087
This data as json
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| 66087 | SRR15900241 | SRX12190887 | SRS10168724 | SRP337081 | PRJNA763243 | m6A RNA methylation regulated by AHR contributes to the cardiac developmental toxicity of PM2.5 in zebrafish embryos | PRJNA763243 | Other | This study aims to investigate the roles of m6A RNA methylation regulated by AHR in the heart developmental toxicity of PM2.5 in zebrafish embryos.EOM downregulates the expression levels of methyltransferase METTL14 which can be restored by AHR inhibitor CH or AHR knockdown. In addition EOM increased ROS levels and cell apoptosis in the heart region of zebrafish embryos and these can be attenuated by enforced METTL14 overexpression. In conclusion AHR activated by PM2.5 significantly inhibits METTL14 expression which in turn disrupts heart development via oxidative stress and apoptosis resulting in heart defects in zebrafish embryos. | EOM+CH 1 | strain:AB|isolate:CH1|breed:missing|cultivar:missing|ecotype:missing|age:missing|dev stage:72 hpf organism or animal | RNA m6A methylation of zebrafish | tchenlab CH1 | tchenlab CH1 | M6A RNA immunoprecipitation was performed using GenSeqTM m6A RNA IP kit GenSeq.NEBNextUltra II Directional RNA Library Prep kit was used to construct RNA sequencing libraries for Input RNA samples without xxx and IP RNA samples post immunoprecipitation.Library inspection is performed using the BioAnalyzer 2100 analyzer Agilent.High throughput sequencing was performed using a 150 bp dual terminal mode on Illumina NovaSeq 6000 sequencer. | RIP-Seq | TRANSCRIPTOMIC | PCR | SINGLE | ILLUMINA | Illumina NovaSeq 6000 | SRP337081 | loader:fastq load.py | EOM_CH_4.16.IP_R1.fastq.gz EOM_CH_4.16.IP_R2.fastq.gz EOM_CH_4.16.Input_R1.fastq.gz EOM_CH_4.16.Input_R2.fastq.gz | fastq fastq fastq fastq | 12428976000.0 | 41429920.0 | EOM CH 4.16.IP R1.fastq.gz | 0:150 1:150 | A:2486344612;C:3648199763;G:3865825828;T:2365039093;N:63566704 | 150 | 150 | 2486344612 | 3648199763 | 3865825828 | 2365039093 | 63566704 | SRX12190887 | SRS10168724 | SRA1294636 | Soochow University|Department of Toxicology, School of Public Health | Soochow University | 2 | 0.91763 | 0.6223 | 0.18817 | 0.15102 | 0.84891 | 0.87957 | 0.74757 | 0.64429 | 150 | 150 | B | B | mate2-mate1 similar by mapping diff | illumina | novaseq_era | unknown | random_priming | nebnext | bulk | unknown | unknown | Unknown | 2021-09-15 | Larval | Larval | Undetermined | Undetermined |