run_metadata: 65969
This data as json
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| 65969 | SRR15712667 | SRX12008335 | SRS10014485 | SRP335554 | PRJNA760458 | adult zebrafish kidney cells | GSE183382 | Other | To illustrate the functions of the various cell types in the zebrafish kidney we sequenced the kidney cells by single cell messenger RNA sequencing. Six randomly selected zebrafish kidneys were used and obtained about 7 147 cells for RNA sequencing using a modified version of the cell expression by linear amplification and sequencing CEL seq method and incorporating unique molecular identifiers to count the transcripts. Overall design: the type of zebrafish kidney cells | pubmed:36645741 | zebrafish kidney cells | GSM5556587 | source name:adult zebrafish kedney cells|strain:AB|tissue:kidney|developmental stage:6 month | zebrafish kidney cells | Sequencing was performed with Illumina NovaSeq according to the manufacturer’s instructions Illumina The Seurat package was used to normalize data dimensionality reduction clustering differential expression. Genome build: danRer11 Supplementary files format and content: Matrix table with raw gene counts for every gene | adult zebrafish kedney cells | Kidney cells from 6 randomly selected zebrafish was loaded into Chromium microfluidic chips with 30 v chemistry. sequencing libraries constructed with reagents from a Chromium Single Cell 30 v3 reagent kit 10X Genomics according to the manufacturer’s instructions 10× Chromium Controller 10X Genomics | Zebrafish were maintained in standard conditions under a 14 hours light and 10 hours dark cycle and fed three times daily | strain:AB|tissue:kidney|developmental stage:6 month | GSM5556587 | GSM5556587: zebrafish kidney cells; Danio rerio; RNA Seq | GSM5556587 | 1 | Kidney cells from 6 randomly selected zebrafish was loaded into Chromium microfluidic chips with 30 v chemistry. sequencing libraries constructed with reagents from a Chromium Single Cell 30 v3 reagent kit 10X Genomics according to the manufacturer's instructions 10× Chromium Controller 10X Genomics | GEO Accession:GSM5556587 | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP335554 | six2_rds-12_S1_L004_R1_001.fastq.gz six2_rds-12_S1_L004_R2_001.fastq.gz | fastq fastq | 13212121800.0 | 44040406.0 | GSM5556587 r12 | 0:150 1:150 | A:5196898881;C:2253733071;G:2178536712;T:3582512811;N:440325 | 150 | 150 | 5196898881 | 2253733071 | 2178536712 | 3582512811 | 440325 | SRX12008335 | SRS10014485 | SRA1288506 | GEO | Xinqiao Hospital, Army Medical University (Third Military Medical University) | 2 | 0.0 | 0.87751 | 0.0 | 0.21742 | 1.0 | 0.79555 | 0.57537 | 150 | 150 | T | B | mate1 technical by mapping diff | illumina | novaseq_era | unknown | cdna_unspecified | unknown | sc | single_cell_droplet | 10x | China | 2021-09-03 | Adult | Adult | Kidney | Renal System |