run_metadata: 65670
This data as json
| rowid | run.accession | experiment.accession | sample.accession | study.accession | bioproject | study.title | study.alias | study.type | study.abstract | study.attributes | study.PMIDs | sample.description | sample.title | sample.alias | sample.centername | sample.attributes | GEOsample.title | GEOsample.dataprocessing | GEOsample.source | GEOsample.treatmentprotocol | GEOsample.extractprotocol | GEOsample.growthprotocol | GEOsample.characteristics | GEOsample.accession | experiment.title | experiment.alias | experiment.library_name | experiment.design_description | experiment.library_construction_protocol | experiment.attributes | experiment.library_strategy | experiment.library_source | experiment.library_selection | experiment.library_layout | experiment.platform | experiment.instrument_model | experiment.spot_descriptor | experiment.study_ref | run.title | run.attributes | run.filename | run.semantic_name | run.total_bases | run.total_spots | run.alias | run.read_lengths | run.base_counts | run.r1_length | run.r2_length | run.r3_length | run.r4_length | run.Acount | run.Ccount | run.Gcount | run.Tcount | run.Ncount | run.experiment | run.pool_member | submission.accession | submission.srasource | submission.bioprojectsource | seqdetective.n_mates | seqdetective.mapping_rate.mate1 | seqdetective.mapping_rate.mate2 | seqdetective.nofeature_rate.mate1 | seqdetective.nofeature_rate.mate2 | seqdetective.sparsity.mate1 | seqdetective.sparsity.mate2 | seqdetective.pos_strand_rate.mate1 | seqdetective.pos_strand_rate.mate2 | seqdetective.readlen.mate1 | seqdetective.readlen.mate2 | seqdetective.judgement.mate1 | seqdetective.judgement.mate2 | seqdetective.judgement.reason | platform_family | instrument_generation | read_bias | selection_class | prep_kit | sc_or_bulk | tech_class | technology | tech_variant | submission.bioprojectsource.country | earliest_date | devstage_curation | devstage_curation_coarse | tissue_curation | tissue_curation_coarse |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 65670 | SRR15498139 | SRX11797852 | SRS9797788 | SRP332839 | PRJNA755482 | miRNA sequencing from zebrafish testes and ovaries exposed to normal and high temperatures | PRJNA755482 | Other | We exposed zebrafish to elevated temperatures during early development a treatment that is known to result in male skewed sex ratios in order to understand the role of the epigenetic regulation mediated by miRNAs in the gonads. | Ovary High Temperature | ZF 36 G9F | strain:AB|age:90 dpf|sex:female|tissue:Ovary|replicate:Biological replicate OHT 1|BioSampleModel:Model organism or animal | miRNA seq of Danio rerio: adult treated ovary | 36 G9 | 36 G9 | NEBNext Small RNA Library Prep Set for Illumina was used on total RNA isolated from zebrafish ovary using Qiagen RNeasy mini kit. Library was constructed using sequencing Lane 1x50 v4 HiSeq single end mode with a read length of 50 bp | miRNA-Seq | TRANSCRIPTOMIC | RT-PCR | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP332839 | ZF_36_G9_22313_ACTGAT.fastq ZF_36_G9_24794_ACTGAT.fastq | fastq fastq | 448040450.0 | 8960809.0 | ZF 36 G9 22313 ACTGAT.fastq | 0:50 1:0 | A:134152732;C:98754272;G:116124383;T:98987716;N:21347 | 50 | 0 | 134152732 | 98754272 | 116124383 | 98987716 | 21347 | SRX11797852 | SRS9797788 | SRA1279325 | Institute of Marine Sciences|Repro-Immune Team | Institute of Marine Sciences | 1 | 0.73407 | 0.52835 | 0.83092 | 0.53201 | 50 | B | usable mapping rate | illumina | hiseq_era | unknown | small_rna | nebnext | bulk | unknown | unknown | United States | 2021-08-17 | Adult | Adult | Gonad | Reproductive System |