run_metadata: 64127
This data as json
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 64127 | SRR14272173 | SRX10633815 | SRS8730197 | SRP315319 | PRJNA722925 | Reinforcing one carbon metabolism via folic acid/Folr1 promotes beta cell differentiation | PRJNA722925 | Other | Diabetes can be caused by an insufficiency in beta cell mass. Here we performed a genetic screen in a zebrafish model of beta cell loss to identify pathways promoting beta cell regeneration. We found that both folate receptor 1 folr1 overexpression and treatment with folinic acid stimulated beta cell differentiation in zebrafish. Treatment with folinic acid also stimulated beta cell differentiation in cultures of neonatal pig islets showing that the effect could be translated to a mammalian system. In both zebrafish and neonatal pig islets the increased beta cell differentiation originated from ductal cells. Mechanistically comparative metabolomic analysis of zebrafish with/without xxx cell ablation and with/without xxx acid treatment indicated beta cell regeneration could be attributed to changes in the pyrimidine carnitine and serine pathways. Overall our results suggest evolutionarily conserved and previously unknown roles for folic acid and one carbon metabolism in the generation of beta cells. | Pancreas islets Embryo 4dpf beta cell ablation B1 | Pancreas islets Emb b cell abt 4d B1 AGN000907 | strain:TU/AB|age:not applicable|dev stage:Day 4|sex:pooled male and female|tissue:embryo|treatment:metronidazole|molecule:mRNA|selection:pA|sample ref:AGS000682|replicate ref:AGN000907|replicate order:1|BioSampleModel:Model organism or animal | Pancreas islets Embryo 4dpf beta cell ablation B1 | AGR001078 | AGR001078 | mRNA | RNA-Seq | TRANSCRIPTOMIC | unspecified | SINGLE | ILLUMINA | Illumina HiSeq 2000 | SRP315319 | AGR001078_R1.fastq.gz | fastq | 686122452.0 | 9027927.0 | AGR001078 R1.fastq.gz | 0:76 1:0 | A:196244052;C:132201159;G:130878329;T:226758661;N:40251 | 76 | 0 | 196244052 | 132201159 | 130878329 | 226758661 | 40251 | SRX10633815 | SRS8730197 | SRA1220217 | Yale_Giraldez|Genetics | Yale_Giraldez_Group | 1 | 0.89799 | 0.26844 | 0.7236 | 0.49736 | 76 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | United States | 2021-04-19 | Larval | Larval | Embryo Imprecise | All anatomical structures |