run_metadata: 63793
This data as json
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 63793 | SRR14055361 | SRX10431250 | SRS8565305 | SRP311888 | PRJNA716463 | Mutation of smarca5 in zebrafish leads to venous thrombosis like phenotype | PRJNA716463 | Other | Our study using zebrafish smarca5 mutants both characterizes a novel role for smarca5 in blood clot formation and also provides a new venous thrombosis animal model to support drug screening and pre clinical therapeutic assessments of therapies to treat thrombosis. | Model organism or animal sample from Danio rerio | RNA RBC sibling rep1 | strain:gata1:dsRed transgenic line|dev stage:2 dpf|sex:not collected|tissue:Blood|BioSampleModel:Model organism or animal | RNA RBC sibling rep1 | RNA RBC sibling rep1 | RNA RBC sibling rep1 | PolyA RNA from 100 200ng total RNA in sorted RBCs from smarca5 siblings and mutants at 2 dpf were used to generate the cDNA libraries respectively | RNA-Seq | TRANSCRIPTOMIC | PCR | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP311888 | 1049RBC-sib-1_FRAS190319999-1a_1.fq.gz 1049RBC-sib-1_FRAS190319999-1a_2.fq.gz | fastq fastq | 10945070100.0 | 36483567.0 | 1049RBC sib 1 FRAS190319999 1a 1.fq.gz | 0:150 1:150 | A:2787518303;C:2738688307;G:2737440984;T:2681219061;N:203445 | 150 | 150 | 2787518303 | 2738688307 | 2737440984 | 2681219061 | 203445 | SRX10431250 | SRS8565305 | SRA1209808 | Tsinghua university|School of Life Sciences | Tsinghua university | 2 | 0.94824 | 0.94818 | 0.04162 | 0.04125 | 0.80247 | 0.80284 | 0.44015 | 0.43533 | 150 | 150 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2021-03-24 | Hatching | Embryo | Blood | Hematopoietic System |