run_metadata: 63792
This data as json
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 63792 | SRR14055360 | SRX10431251 | SRS8565306 | SRP311888 | PRJNA716463 | Mutation of smarca5 in zebrafish leads to venous thrombosis like phenotype | PRJNA716463 | Other | Our study using zebrafish smarca5 mutants both characterizes a novel role for smarca5 in blood clot formation and also provides a new venous thrombosis animal model to support drug screening and pre clinical therapeutic assessments of therapies to treat thrombosis. | Model organism or animal sample from Danio rerio | RNA RBC sibling rep2 | strain:gata1:dsRed transgenic line|dev stage:2 dpf|sex:not collected|tissue:Blood|BioSampleModel:Model organism or animal | RNA RBC sibling rep2 | RNA RBC sibling rep2 | RNA RBC sibling rep2 | PolyA RNA from 100 200ng total RNA in sorted RBCs from smarca5 siblings and mutants at 2 dpf were used to generate the cDNA libraries respectively | RNA-Seq | TRANSCRIPTOMIC | PCR | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP311888 | 1049RBC-sib-2_FRAS190320000-1a_1.fq.gz 1049RBC-sib-2_FRAS190320000-1a_2.fq.gz | fastq fastq | 10846357200.0 | 36154524.0 | 1049RBC sib 2 FRAS190320000 1a 1.fq.gz | 0:150 1:150 | A:2755761785;C:2720527184;G:2723864022;T:2645998029;N:206180 | 150 | 150 | 2755761785 | 2720527184 | 2723864022 | 2645998029 | 206180 | SRX10431251 | SRS8565306 | SRA1209808 | Tsinghua university|School of Life Sciences | Tsinghua university | 2 | 0.94927 | 0.94734 | 0.04134 | 0.04034 | 0.80505 | 0.80586 | 0.43562 | 0.43266 | 150 | 150 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2021-03-24 | Hatching | Embryo | Blood | Hematopoietic System |