run_metadata: 63791
This data as json
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 63791 | SRR14055359 | SRX10431252 | SRS8565307 | SRP311888 | PRJNA716463 | Mutation of smarca5 in zebrafish leads to venous thrombosis like phenotype | PRJNA716463 | Other | Our study using zebrafish smarca5 mutants both characterizes a novel role for smarca5 in blood clot formation and also provides a new venous thrombosis animal model to support drug screening and pre clinical therapeutic assessments of therapies to treat thrombosis. | Model organism or animal sample from Danio rerio | RNA RBC sibling rep3 | strain:gata1:dsRed transgenic line|dev stage:2 dpf|sex:not collected|tissue:Blood|BioSampleModel:Model organism or animal | RNA RBC sibling rep3 | RNA RBC sibling rep3 | RNA RBC sibling rep3 | PolyA RNA from 100 200ng total RNA in sorted RBCs from smarca5 siblings and mutants at 2 dpf were used to generate the cDNA libraries respectively | RNA-Seq | TRANSCRIPTOMIC | PCR | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP311888 | 1049RBC-sib-3_FRAS190320001-1a_1.fq.gz 1049RBC-sib-3_FRAS190320001-1a_2.fq.gz | fastq fastq | 13833315900.0 | 46111053.0 | 1049RBC sib 3 FRAS190320001 1a 1.fq.gz | 0:150 1:150 | A:3515133007;C:3462086735;G:3467124241;T:3388720799;N:251118 | 150 | 150 | 3515133007 | 3462086735 | 3467124241 | 3388720799 | 251118 | SRX10431252 | SRS8565307 | SRA1209808 | Tsinghua university|School of Life Sciences | Tsinghua university | 2 | 0.93805 | 0.93649 | 0.04283 | 0.04244 | 0.80752 | 0.80817 | 0.44958 | 0.44864 | 150 | 150 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2021-03-24 | Hatching | Embryo | Blood | Hematopoietic System |