run_metadata: 63788
This data as json
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 63788 | SRR14055354 | SRX10431257 | SRS8565312 | SRP311888 | PRJNA716463 | Mutation of smarca5 in zebrafish leads to venous thrombosis like phenotype | PRJNA716463 | Other | Our study using zebrafish smarca5 mutants both characterizes a novel role for smarca5 in blood clot formation and also provides a new venous thrombosis animal model to support drug screening and pre clinical therapeutic assessments of therapies to treat thrombosis. | Model organism or animal sample from Danio rerio | RNA RBC mutant rep3 | strain:gata1:dsRed transgenic line|dev stage:2 dpf|sex:not collected|tissue:Blood|BioSampleModel:Model organism or animal | RNA RBC mutant rep3 | RNA RBC mutant rep3 | RNA RBC mutant rep3 | PolyA RNA from 100 200ng total RNA in sorted RBCs from smarca5 siblings and mutants at 2 dpf were used to generate the cDNA libraries respectively | RNA-Seq | TRANSCRIPTOMIC | PCR | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP311888 | 1049RBC-mu-3_FRAS190320004-1a_1.fq.gz 1049RBC-mu-3_FRAS190320004-1a_2.fq.gz | fastq fastq | 13709534700.0 | 45698449.0 | 1049RBC mu 3 FRAS190320004 1a 1.fq.gz | 0:150 1:150 | A:3530756610;C:3372588726;G:3372560453;T:3433404064;N:224847 | 150 | 150 | 3530756610 | 3372588726 | 3372560453 | 3433404064 | 224847 | SRX10431257 | SRS8565312 | SRA1209808 | Tsinghua university|School of Life Sciences | Tsinghua university | 2 | 0.92707 | 0.92658 | 0.05411 | 0.05405 | 0.75607 | 0.75568 | 0.45262 | 0.45118 | 150 | 150 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2021-03-24 | Hatching | Embryo | Blood | Hematopoietic System |