run_metadata: 61877
This data as json
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| 61877 | SRR13039580 | SRX9489631 | SRS7698470 | SRP292270 | PRJNA677856 | Investigation of myocardial retionic acid signaling during heart regeneration in zebrafish | PRJNA677856 | Other | Retinoic acid RA signaling is required for heart regeneration in zebrafish but the mechanism of RA mediated cardiomyocyte proliferation remains unclear. To investigate the downstream mechanism of RA mediated cardiomyocyte proliferation we generated a new transgenic line in which a loxP flanked stop cassette blocks the expression of a dominant negative form of RA receptor a b actin2:LSL dn rara and crossed this strain with a cardiomyocyte specific inducible Cre driver line cmlc2:CreER. We performed ventricular resection surgery on cmlc2:CreER; b actin2:LSL dn rara fish with and without xxx inhibition and collected 3 and 7 xxx post injury hearts to compare the gene expression profile of regenerating hearts. This project contains raw sequencing data sets of these hearts that were used to identify genes of which expression is regulated by RA signaling during regeneration. | dnRAR | strain:dnRAR|dev stage:adult|sex:pooled male and female|tissue:heart|genotype:dnRAR|sample type:tissue sample|BioSampleModel:Model organism or animal | dnRAR pos injury 7d prep1 | dnRAR 7dpi .prep1 | dnRAR pos 7dpi 1 | Bulk RNA Seq polyA adult zebrafish hearts | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2500 | SRP292270 | loader:fastq load.py | 7dpi_dnrar_pos_1__Index_7__CAGATC_L001_R1.fastq.gz 7dpi_dnrar_pos_1__Index_7__CAGATC_L002_R1.fastq.gz | fastq fastq | 3132430463.0 | 31014163.0 | 7dpi dnrar pos 1 Index 7 CAGATC L001 R1.fastq.gz | 0:101 | A:853203437;C:688250700;G:670822036;T:919154522;N:999768 | 101 | 853203437 | 688250700 | 670822036 | 919154522 | 999768 | SRX9489631 | SRS7698470 | SRA1157756 | Victor Chang Cardiac Research Institute|Genome core | Victor Chang Cardiac Research Institute | 1 | 0.93753 | 0.11165 | 0.74176 | 0.50574 | 101 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | unknown | bulk | bulk | bulk | Australia | 2020-11-16 | Adult | Adult | Heart | Cardiovascular System |