run_metadata: 61623
This data as json
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| 61623 | SRR12890228 | SRX9355399 | SRS7578435 | SRP288489 | PRJNA671696 | DAPT dependent regulation of zebrafish Muller glial cell gene expression | GSE160047 | Transcriptome Analysis | In this work we identify genes differentially expressed in Muller glia from uninjured retina in zebrafish treated with and without xxx for 1 day. Overall design: Triplicate samples from control DMSO treated and experimental DAPT treated gfap:GFP transgenic zebrafish. | parent bioproject:PRJNA672149 | pubmed:34415582 | DMSO 2 | GSM4855906 | tissue:Mueller Glia cells|treatment:DMSO | DMSO 2 | Alignment with TopHat v2.0.13 and bowtie2 v2.2.1 with b2 very sensitive htseq count v0.6.1 Genome build: danRer10 Supplementary files format and content: tab delimited text of raw counts | Mueller Glia cells | Fish were immersed in fish water containing DMSO or DAPT | One day post treatment retinas were isolated dissociated and GFP+ Muller glia purified by FACS. RNA was isolated with Trizol. RNA libraries prepared using standard Illumina protocols | Zebrafish were maintained in aquaria at 28 degrees centrigrade | treatment:DMSO | GSM4855906 | GSM4855906: DMSO 2; Danio rerio; RNA Seq | GSM4855906 | 1 | One day post treatment retinas were isolated dissociated and GFP+ Muller glia purified by FACS. RNA was isolated with Trizol. RNA libraries prepared using standard Illumina protocols | GEO Accession:GSM4855906 | RNA-Seq | TRANSCRIPTOMIC | cDNA | SINGLE | ILLUMINA | Illumina HiSeq 4000 | SRP288489 | 121566_GACCTGAA-TTGGTGAG_S2_L003_R1_001.fastq.gz | fastq | 3264529941.0 | 64010391.0 | GSM4855906 r1 | 0:51 1:0 | A:923700093;C:710395473;G:705063079;T:924792878;N:578418 | 51 | 0 | 923700093 | 710395473 | 705063079 | 924792878 | 578418 | SRX9355399 | SRS7578435 | SRA1147626 | GEO | University of Michigan | 1 | 0.88907 | 0.23013 | 0.72123 | 0.48896 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | cdna_unspecified | unknown | bulk | unknown | unknown | United States | 2020-10-25 | Undetermined | Undetermined | Eye | Sensory System |