run_metadata: 60149
This data as json
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| 60149 | SRR12201311 | SRX8712178 | SRS6988102 | SRP270158 | PRJNA644214 | Danio rerio Transcriptome under lethal cold stress | PRJNA644214 | Transcriptome Analysis | The transcriptional regulations of fish upon a lethal cold stress and during the following rewarming are not well known. The purpose of this study is to characterize the gene expression dynamics of zebrafish larvae exposed to lethal cold stress and subsequent rewarming. Our data defined the survival and death pathways determining the survival of fish from the cold induced cellular or tissue damage. Modulating the activities of these pathways may enhance cold tolerance of the cold sensitive farmed fishes. | Zebrafish larvae exposed to lethal cold stress for xxxh followed by 6h recovery at normal temperature replicate #3 | re 6h#3 | re 6h#3 | strain:AB|dev stage:Larvae|sex:not applicable|tissue:Whole body|biomaterial provider:Zongbin Cui No. 7 Donghu South Road Wuchang District Wuhan Hubei Province China|treatment:recovery 6h|replicate:biological replicate 3|BioSampleModel:Model organism or animal | re 6h#3 | re 6h#3 | re 6h#3 | The RNA sample was sent to Biomarker Technologies http://www.biomarker.com.cn for library construction and RNA sequencing. Briefly mRNA molecules were enriched using Oligo dT magnetic beads and randomly fragmented. First strand cDNA synthesis was performed using random hexamers. Second strand cDNAs were synthesized by adding dNTPs RNase and DNA polymerase. The double stranded cDNAs were purified using AMPure XP beads. post end filling A tailing and sequencing adaptor ligation cDNA fragments of desired size were selected using AMPure XP beads. Finally the sequencing library was generated by PCR amplification. Library quantification was performed using a Qubit 2.0 and the insert size of library was analyzed by an Agilent 2102 Bioanalyzer system. | RNA-Seq | TRANSCRIPTOMIC | PCR | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP270158 | Zebrafish_N082-01-T109_good_1.fq.gz Zebrafish_N082-01-T109_good_2.fq.gz | fastq fastq | 8656222216.0 | 29119374.0 | Zebrafish N082 01 T109 good 1.fq.gz | 0:148.63 1:148.63 | A:2224288504;C:2107136529;G:2119597196;T:2205191367;N:8620 | 148 | 148 | 2224288504 | 2107136529 | 2119597196 | 2205191367 | 8620 | SRX8712178 | SRS6988102 | SRA1097851 | Chinese Academy of Sciences|Institute of Hydrobiology | Chinese Academy of Sciences | 2 | 0.94918 | 0.9507 | 0.06678 | 0.06714 | 0.65105 | 0.65109 | 0.46125 | 0.46263 | 150 | 150 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2020-07-11 | Larval | Larval | Trunk | Surface Structure |