run_metadata: 59094
This data as json
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 59094 | SRR11590437 | SRX8157786 | SRS6519796 | SRP257942 | PRJNA627467 | Fli1+ cells transcriptional analysis reveals LMO2 PRDM16 axis in angiogenesis | GSE149152 | Transcriptome Analysis | Different GFP+ cell population were isolated from Tgfli1:EGFP zebrafish embryos. Novel roles of epigenetic modifiers in endothelial differentiation were identified. Overall design: Each sample has been run in duplicate with 2 files per each run | pubmed:34330825 | High Fli1+ rep1 | GSM4491075 | tissue:High Fli1+|cell population:High Fli1+|genotype:Tgfli1:EGFP | High Fli1+ rep1 | Illumina Illumina Hi Seq 2500 software used for basecalling. Sequenced reads were trimmed for adaptor sequence and masked for low complexity or low quality sequence then mapped to dr10 whole genome Fragments Per Kilobase of exon per Megabase of library size FPKM were calculated. Genome build: danio rerio10 Supplementary files format and content: Text files with FPKM values for each sample were grouped in a single excel file | High Fli1+ | 100 ng of total RNA was subjected to library synthesis Total RNA was enriched for the Poly A mRNA and reverse transcribed to double stranded cDNA. | cell population:High Fli1+|genotype:Tgfli1:EGFP | GSM4491075 | GSM4491075: High Fli1+ rep1; Danio rerio; RNA Seq | GSM4491075 | 1 | 100 ng of total RNA was subjected to library synthesis Total RNA was enriched for the Poly A mRNA and reverse transcribed to double stranded cDNA. | GEO Accession:GSM4491075 | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | ILLUMINA | Illumina HiSeq 2500 | SRP257942 | CI5705_ACTTGA_L005_R1_001.fastq.gz CI5705_ACTTGA_L005_R2_001.fastq.gz | fastq fastq | 8541050000.0 | 42705250.0 | GSM4491075 r1 | 0:100 1:100 | A:1667536155;C:2542273851;G:2600174460;T:1724878449;N:6187085 | 100 | 100 | 1667536155 | 2542273851 | 2600174460 | 1724878449 | 6187085 | SRX8157786 | SRS6519796 | SRA1068019 | GEO | University of Edinburgh | 2 | 0.92757 | 0.93319 | 0.14438 | 0.14609 | 0.78867 | 0.79346 | 0.72736 | 0.71784 | 100 | 100 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | bulk | bulk | bulk | United Kingdom | 2020-04-22 | Undetermined | Embryo | Undetermined | Embryo Imprecise |