run_metadata: 58565
This data as json
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 58565 | SRR11411804 | SRX7990456 | SRS6374486 | SRP253956 | PRJNA615045 | Strategy of Hepatic Metabolic Defects Induced by beclin1 Heterozygosity in Adult Zebrafish | PRJNA615045 | Other | In our study mutant zebrafish lines of autophagy related genes beclin1 were generated by CRISPR/Cas9 gene editing technology.Interestingly male beclin1 heterozygous zebrafish displayed liver defects in the morphology and histology including abnormal hepatocyte proliferation hemorrhagic and inflammatory phenotypes. In order to better study the phenotype of beclin1 heterozygous zebrafish we extracted RNA from the liver of 12 mpf male beclin1 heterozygous and WT for transcriptomics research. | zebrafish | strain:missing|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:12months|dev stage:sex mature|sex:male|tissue:liver|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio: adult male liver | beclin1 heterozygous | beclin1 heterozygous | Sequencing libraries were generated using the NEBNext Ultra RNA Library Prep Kit for Illumina #E7530L NEB USA following the manufacturers recommendations | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP253956 | Beclin1_R1.fq.gz Beclin1_R2.fq.gz | fastq fastq | 7286695500.0 | 24288985.0 | Beclin1 R1.fq.gz | 0:150 1:150 | A:1901077388;C:1739767288;G:1753568948;T:1892271367;N:10509 | 150 | 150 | 1901077388 | 1739767288 | 1753568948 | 1892271367 | 10509 | SRX7990456 | SRS6374486 | SRA1059106 | Huazhong Agricultural University|College of Fisheries | Huazhong Agricultural University | 2 | 0.9568 | 0.95511 | 0.03708 | 0.03692 | 0.78348 | 0.78352 | 0.47534 | 0.47335 | 150 | 150 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | nebnext | bulk | unknown | unknown | China | 2020-03-25 | Adult | Adult | Liver | Liver and Biliary System |