run_metadata: 57272
This data as json
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| 57272 | SRR12577971 | SRX9064852 | SRS7314025 | SRP279881 | PRJNA612371 | Danio rerio strain:TU Raw sequence reads | PRJNA612371 | Whole Genome Sequencing | Hemorrhage stroke is a severe vascular disease of the brain with a high mortality rate in humans. Sodium tanshinone IIA sulfonate STS is a water soluble derivative of tanshinone IIA which is the main active ingredient of Salvia miltiorrhiza Bge known as Danshen in Chinese and has been approved as a commercial drug for treating cardiovascular disease by the China Food and Drug Administration. In our previous study we established a HMG COA inhibitor atorvastatin Ator induced zebrafish model of cerebral hemorrhage and found that STS dramatically decreased both the hemorrhage rate and hemorrhage area although the underlying mechanism was not fully elucidated. Therefore in the present study we conducted transcriptome analysis of the protective effect of STS against Ator induced cerebral hemorrhage in zebrafish using RNA Seq technology and further clarify its underlying molecular mechanisms on HIF 1 and its regulators i.e. the PI3K/Akt and MAPK signaling pathways were verified by real time PCR analysis and specific pharmacological inhibitors. We are also able to show that hemoglobin carbonic anhydrase Na+/H+ exchanger and HIF 1 genes might be potential biomarkers of Ator induced cerebral hemorrhage in zebrafish as well as pharmacological targets of STS. This study also provided evidence of bio markers involved in hemorrhage stroke and improved understanding of the effects of HMG COA inhibition on vascular permeability and cerebral hemorrhage. | Ctrl 2 zebrafish | strain:TU|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:2dpf|dev stage:not collected|sex:not applicable|tissue:head|geo loc name:China:Shanghai|sample type:model organism|replicate:replicate=biological replicate 5 prime|BioSampleModel:Model organism or animal | Ctrl 2 zebrafish | Ctrl 2 zebrafish | Ctrl 2 zebrafish | transcriptome | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | BGISEQ | BGISEQ-500 | SRP279881 | Ctrl_2_1.fq.gz | fastq | 1058116700.0 | 21162334.0 | Ctrl 2 1.fq.gz | 0:50 | A:287223905;C:238707750;G:244987559;T:287197486;N:0 | 50 | 287223905 | 238707750 | 244987559 | 287197486 | 0 | SRX9064852 | SRS7314025 | SRA1120721 | Shanghai University of Traditional Chinese Medicine|Longhua Hospital | Shanghai University of Traditional Chinese Medicine | 1 | 0.94534 | 0.11174 | 0.70104 | 0.47946 | 50 | B | usable mapping rate | bgi | bgi | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2020-09-03 | Hatching | Embryo | Head | Nervous System |