run_metadata: 55327
This data as json
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| 55327 | SRR10277634 | SRX6990843 | SRS5512852 | SRP225662 | PRJNA577580 | Dynamic Properties of the Reissner Fiber and Implications on Scoliosis | GSE138842 | Transcriptome Analysis | Background: RNASeq of a scospondin hypomorphic mutant at differnet time points Overall design: RNASeq of a scospondin hypomorphic mutant at differnet time points | 30dpf scospondin stl297 maternal zygotic mutant Library 3 | GSM4120307 | tissue:30dpf scospondin stl297 maternal zygotic mutant Library 3|cell type:WHOLE BODY|iPSc source:N/A|passage:N/A | 30dpf scospondin stl297 maternal zygotic mutant Library 3 | Raw reads were trimmed with cutadapt to remove low quality bases Trimmed reads were aligned to danio rerio genome GRCz10 using STAR 2.5.4b with Ensembl v83 annotation Aligned reads were quantified using featureCounts 1.6.3 from the subreads package Genome build: GRCz10 Supplementary files format and content: gene counts text file | 30dpf scospondin stl297 maternal zygotic mutant Library 3 | None | Trizol isolation of RNA; Clean up on QIAGEN RNA column. Takara Clontech SMARTer prep | Typical | cell type:WHOLE BODY|iPSc source:N/A|passage:N/A | GSM4120307 | GSM4120307: 30dpf scospondin stl297 maternal zygotic mutant Library 3; Danio rerio; RNA Seq | GSM4120307 | 1 | Trizol isolation of RNA; Clean up on QIAGEN RNA column. Takara Clontech SMARTer prep | GEO Accession:GSM4120307 | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP225662 | stl297_homoz_30d_r3_1.fastq.gz stl297_homoz_30d_r3_2.fastq.gz | fastq fastq | 8251283260.0 | 27322130.0 | GSM4120307 r1 | 0:151 1:151 | A:2171660201;C:1843866593;G:2178007804;T:2057620716;N:127946 | 151 | 151 | 2171660201 | 1843866593 | 2178007804 | 2057620716 | 127946 | SRX6990843 | SRS5512852 | SRA979154 | GEO | Zhang, Developmental Biology, Washington University School of Medicine | 2 | 0.93766 | 0.94573 | 0.06206 | 0.06453 | 0.71086 | 0.71857 | 0.48398 | 0.48567 | 151 | 151 | B | B | biological fallback assumption | illumina | novaseq_era | full_length | cdna_unspecified | smarter | bulk | unknown | unknown | United States | 2019-10-15 | Juvenile | Juvenile | Trunk | Surface Structure |