run_metadata: 53511
This data as json
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| 53511 | SRR9899211 | SRX6651054 | SRS5212730 | SRP217299 | PRJNA558506 | Bmpr2b mutant zebrafish sequencing | PRJNA558506 | Other | In order to explore the differential expression genes and related pathways caused by bmpr2b deletion in zebrafish. The comparative transcriptome analysis between wild and mutant samples was conducted. | zebrafish bmpr2b mutant | strain:AB zebrafish|isolate:mutant|breed:zebrafish|cultivar:zebrafish|ecotype:Wuhan China|age:15 days|dev stage:larval fish|sex:unknown|tissue:skeleton muscle|BioSampleModel:Model organism or animal | RNA Seq of mutant fish | mutant3 | mutant3 | Total RNA was isolated from the 15 dpf dpf bmpr2b mutant larval fishes using RNAiso Plus Reagent TaKaRa China according to the manufacturers protocol. post RNA quality and quantity control the transcriptome equencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina NEB USA following manufacturers recommendations and index codes were added to attribute sequences to each sample. | RNA-Seq | TRANSCRIPTOMIC | size fractionation | PAIRED | ILLUMINA | Illumina HiSeq 2000 | SRP217299 | m3_1.fq.gz m3_2.fq.gz | fastq fastq | 8385335400.0 | 27951118.0 | m3 1.fq.gz | 0:150 1:150 | A:2179183324;C:2013493686;G:2044351918;T:2148211353;N:95119 | 150 | 150 | 2179183324 | 2013493686 | 2044351918 | 2148211353 | 95119 | SRX6651054 | SRS5212730 | SRA932406 | Huazhong Agricultural University|College of Fisheries, Key Lab of Freshwater Animal | Huazhong Agricultural University | 2 | 0.94884 | 0.94904 | 0.0643 | 0.06403 | 0.66145 | 0.66316 | 0.46951 | 0.4767 | 150 | 150 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | size_fractionation | nebnext | bulk | unknown | unknown | China | 2019-08-03 | Larval | Larval | Muscle | Muscular System |