run_metadata: 52149
This data as json
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| 52149 | SRR8953738 | SRX5733571 | SRS4646935 | SRP193814 | PRJNA535392 | Transcriptome analysis and identification of insecticide metabolism related genes post exposure to insecticide in Sitobion avenae | PRJNA535392 | Other | Aphids causes serious lost in the production of wheat. Grain aphid Sitobion avenae is the dominant species of aphid in all wheat region of China and this species is also considered resistant to a variety of insecticides including imidacloprid and chlorpyrifos. However the resistance and metabolize mechanism of insecticides for S. avenae is still unclear. This study employed transcriptome analysis to compare the expression patterns of stress response genes under imidacloprid and chlorpyrifos for 15min 3h and 36h'exposure. post compared insecticide treated samples of different time duration to control sample we obtained 60 to 2267 Differential Express Unigenes DEUs among these DEUs 31 790 unigenes was classified into 66 786 categories of GO function group and 24 to 760 DEUs could be mapped into 54 to 268 KEGG pathways. The expression of DEUs related to insecticide metabolism related genes were analyzed. In the insecticide metabolism related genes cuticle protein is the largest group in DEUs and the second largest is ABC transporter. Our study will facilitate molecular research on insecticide resistance in S. avenae as well as in other wheat aphids. | miR 430 / embryos were injected with miR 430 rescued 20 embryos at shield stage were collected for RNA seq analsysis | Rescued | R6 | strain:AB|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:Hong Kong|age:6 hpf stage:shield stage|sex:pooled male and female|tissue:embyos|phenotype:MiR 430 / |sample type:embryos|treatment:Rescued by miR 430 injection|BioSampleModel:Model organism or animal | Transcriptome analysis and identification of insecticidemetabolism related genes post exposure to insecticide in Sitobion avenae | IM15min 1 | IM15min 1 | IM15min 1 | RNA-Seq | TRANSCRIPTOMIC | PCR | PAIRED | ILLUMINA | HiSeq X Ten | SRP193814 | IM15min_1_S91_L004_R1_001.fastq.gz IM15min_1_S91_L004_R2_001.fastq.gz | fastq fastq | 5908953744.0 | 19566072.0 | IM15min 1 S91 L004 R1 001.fastq.gz | 0:151 1:151 | A:1720180168;C:1223518369;G:1218504877;T:1746709378;N:40952 | 151 | 151 | 1720180168 | 1223518369 | 1218504877 | 1746709378 | 40952 | SRX5733571 | SRS4646935 | SRA879464 | Institute of Plant Protection and Agro-Products Safety|Anhui Academy of Agricultural Sciences | Institute of Plant Protection and Agro-Products Safety | 2 | 0.0 | 2e-05 | 0.0 | 0.0 | 1.0 | 0.99995 | 0.33333 | 151 | 151 | T | T | mates < 9% mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2020-01-25 | Gastrula | Embryo | Whole Organism | All anatomical structures |