run_metadata: 50529

This data as json

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50529	SRR8129708	SRX4950830	SRS3993021	SRP167139	PRJNA499073	Purification of high quality RNA from a small number of fluorescence activated cell sorted zebrafish cells for RNA sequencing purposes	GSE121917	Transcriptome Analysis	We provide a method for high quality RNA purification out of a small number 5000 100 000 of FACS sorted zebrafish cells followed by RNA sequencing Overall design: RNA sequencing data from 6 RNA samples isolated from 20000 sorted fli:GFP zebrafish cells. 2 RNA samples isolated from whole embryo.		pubmed:30894119		RNeasy sorted 2 replicate b	GSM3449969		tissue:Fli:GFP sorted 3 days RNeasy sample 2 replicate b\|cell type:fli:GFP GFP sorted\|age:3 days\|rna isolation kit:RNeasy plus micro kit	RNeasy sorted 2 replicate b	Fastq files were aligned to GRCz10 with STAR v2 4 2a. Gene quantification was done on the fly by STAR on Danio rerio.GRCz10.91.gtf Genome build: GRCz10 Supplementary files format and content: tsv files with raw count data	Fli:GFP sorted 3 days RNeasy sample 2 replicate b		polyA RNA cDNA was synthesized according to the SMART seq V4 takara biosystems technology tagmentation and sample barcoding with the Nextera XT kit illumina		cell type:fli:GFP GFP sorted\|age:3 days\|rna isolation kit:RNeasy plus micro kit	GSM3449969	GSM3449969: RNeasy sorted 2 replicate b; Danio rerio; RNA Seq	GSM3449969		1	polyA RNA cDNA was synthesized according to the SMART seq V4 takara biosystems technology tagmentation and sample barcoding with the Nextera XT kit illumina	GEO Accession:GSM3449969	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	NextSeq 500		SRP167139			Q_2b_R1.fastq.gz Q_2b_R2.fastq.gz	fastq fastq	3579768697.0	23697156.0	GSM3449969 r1	0:75.53 1:75.53	A:917613628;C:877326634;G:877017498;T:907801181;N:9756	75	75			917613628	877326634	877017498	907801181	9756	SRX4950830	SRS3993021	SRA800291	GEO	Center for Medical Genetics, Ghent University	2	0.93264	0.93171	0.08657	0.08851	0.73878	0.74383	0.48764	0.4769	75	75	B	B	biological fallback assumption	illumina	nextseq	unknown	poly_a	nextera	sc	single_cell_plate	smartseq		Belgium	2018-10-29	Larval	Larval	Undetermined	Undetermined