run_metadata: 49349
This data as json
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 49349 | SRR7891995 | SRX4729603 | SRS3812040 | SRP162371 | PRJNA492713 | Zebrafish macroH2A variants analysis reveals distinct embryo localization and function RNASeq Information | PRJNA492713 | Other | Most mouse and cell based studies have shown that macroH2A histone variants predominantly associate with heterochromatin and have been associated with gene repression and with an important function in cell differentiation and inhibiting acquisition of pluripotency of pluripotency. However fewer studies have analysed the role of macroH2A during early embryo development. In the project transgenic zebrafish lines were developed expressing macroH2A isoforms mH2A1 and mH2A2 fusion proteins GFP under endogenous identified promoters to study their function during embryogenesis. | mH2A2 75% epiboly GFP sample2 Lane1 | 10.I2 epi gfp 2 Lane1 | strain:TAB.L ABxTL|dev stage:75% epiboly|sex:pooled male and female|tissue:whole embryo without xxx|genotype:TgmH2A2:GFP mH2A2.|samples information:mH2A2 75% epiboly GFP sample2 Lane1|BioSampleModel:Model organism or animal | mH2A2 75% epiboly GFP sample2 Lane1 | 10.I2 epi gfp 2 Lane1 | 10.I2 epi gfp 2 Lane1 | mH2A2 75% epiboly GFP sample2 Lane1 | RNA-Seq | TRANSCRIPTOMIC | PolyA | PAIRED | ILLUMINA | NextSeq 500 | SRP162371 | 10.I2-epi-gfp-2-Lane1-R1.fastq 10.I2-epi-gfp-2-Lane1-R2.fastq | fastq fastq | 283096715.0 | 1877442.0 | 10.I2 epi gfp 2 Lane1 R2.fastq | 0:75.48 1:75.31 | A:71033881;C:69720110;G:70846628;T:71338427;N:157669 | 75 | 75 | 71033881 | 69720110 | 70846628 | 71338427 | 157669 | SRX4729603 | SRS3812040 | SRA782245 | University of Nebraska Lincoln|Electrical and Computer Engineering | University of Nebraska Lincoln | 2 | 0.9661 | 0.96876 | 0.05255 | 0.05163 | 0.75532 | 0.75824 | 0.48277 | 0.48181 | 75 | 76 | B | B | biological fallback assumption | illumina | nextseq | unknown | poly_a | unknown | bulk | unknown | unknown | United States | 2019-10-21 | Gastrula | Embryo | Whole Organism | All anatomical structures |