run_metadata: 49193
This data as json
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| 49193 | SRR7785384 | SRX4640370 | SRS3738588 | SRP159583 | PRJNA489308 | Over expression of EVI 1 oncogene regulate multi pathway to affect zebrafish embryogenesis and hemopoiesis | PRJNA489308 | Other | Our group established Tg Tol GFP EVI 1 zebrafish line and detected survival rate and developmental abnormality in injected embryos. And we found Tg Tol GFP EVI 1 zebrafish embryos underwent abnormal hematopoiesis and aberrant expression of human EVI 1 perturbs hematopoiesis to promote myeloid myeloid and suppress erythroid lineage fates. Moreover overexpression of human EVI 1 induced immature hematopoietic cells emerged in blood circulation. We used whole genome transcriptome analysis RNA Seq to determine differential gene expression in peripheral blood between wildtype larvae and EVI 1 transgenic zebrafish larvae. | H125 | breed:AB strain|age:3 month|sex:male|tissue:Peripheral blood|genotype:Tol2 EVI 1 EGFP transgenic|replicate:replicate2|BioSampleModel:Model organism or animal | H125 | H125 | H125 | Tol2 EVI 1 EGFP transgenic zebrafish sample 2 | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | ILLUMINA | Illumina HiSeq 2500 | SRP159583 | H125_S2_L001_R1_001.fastq.gz H125_S2_L001_R2_001.fastq.gz | fastq fastq | 16012998000.0 | 53376660.0 | H125 S2 L001 R1 001.fastq.gz | 0:150 1:150 | A:4365269809;C:3662731840;G:3768367416;T:4216409682;N:219253 | 150 | 150 | 4365269809 | 3662731840 | 3768367416 | 4216409682 | 219253 | SRX4640370 | SRS3738588 | SRA766519 | Department of Haematology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University|Department: Department of Haematology | Department of Haematology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University | 2 | 0.9402 | 0.94198 | 0.09449 | 0.09528 | 0.71999 | 0.72512 | 0.46106 | 0.47624 | 150 | 150 | B | B | biological fallback assumption | illumina | hiseq_era | full_length | random_priming | unknown | bulk | unknown | unknown | China | 2018-09-04 | Adult | Adult | Blood | Hematopoietic System |