run_metadata: 49191
This data as json
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| 49191 | SRR7785382 | SRX4640372 | SRS3738592 | SRP159583 | PRJNA489308 | Over expression of EVI 1 oncogene regulate multi pathway to affect zebrafish embryogenesis and hemopoiesis | PRJNA489308 | Other | Our group established Tg Tol GFP EVI 1 zebrafish line and detected survival rate and developmental abnormality in injected embryos. And we found Tg Tol GFP EVI 1 zebrafish embryos underwent abnormal hematopoiesis and aberrant expression of human EVI 1 perturbs hematopoiesis to promote myeloid myeloid and suppress erythroid lineage fates. Moreover overexpression of human EVI 1 induced immature hematopoietic cells emerged in blood circulation. We used whole genome transcriptome analysis RNA Seq to determine differential gene expression in peripheral blood between wildtype larvae and EVI 1 transgenic zebrafish larvae. | H93 | breed:AB strain|age:3 month|sex:male|tissue:Peripheral blood|genotype:wild type|replicate:replicate3|BioSampleModel:Model organism or animal | H93 | H93 | H93 | Wild type zebrafish control 3 | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | ILLUMINA | Illumina HiSeq 2500 | SRP159583 | H93_S3_L001_R1_001.fastq.gz H93_S3_L001_R2_001.fastq.gz | fastq fastq | 18197379600.0 | 60657932.0 | H93 S3 L001 R1 001.fastq.gz | 0:150 1:150 | A:4814246240;C:4308418881;G:4375398316;T:4699211054;N:105109 | 150 | 150 | 4814246240 | 4308418881 | 4375398316 | 4699211054 | 105109 | SRX4640372 | SRS3738592 | SRA766519 | Department of Haematology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University|Department: Department of Haematology | Department of Haematology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University | 2 | 0.9312 | 0.93173 | 0.07454 | 0.07498 | 0.67065 | 0.6773 | 0.46807 | 0.47334 | 150 | 150 | B | B | biological fallback assumption | illumina | hiseq_era | full_length | random_priming | unknown | bulk | unknown | unknown | China | 2019-09-20 | Adult | Adult | Blood | Hematopoietic System |