run_metadata: 49188
This data as json
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| 49188 | SRR7785379 | SRX4640375 | SRS3738589 | SRP159583 | PRJNA489308 | Over expression of EVI 1 oncogene regulate multi pathway to affect zebrafish embryogenesis and hemopoiesis | PRJNA489308 | Other | Our group established Tg Tol GFP EVI 1 zebrafish line and detected survival rate and developmental abnormality in injected embryos. And we found Tg Tol GFP EVI 1 zebrafish embryos underwent abnormal hematopoiesis and aberrant expression of human EVI 1 perturbs hematopoiesis to promote myeloid myeloid and suppress erythroid lineage fates. Moreover overexpression of human EVI 1 induced immature hematopoietic cells emerged in blood circulation. We used whole genome transcriptome analysis RNA Seq to determine differential gene expression in peripheral blood between wildtype larvae and EVI 1 transgenic zebrafish larvae. | H67 | breed:AB strain|age:3 month|sex:male|tissue:Peripheral blood|genotype:wild type|replicate:replicate2|BioSampleModel:Model organism or animal | H67 | H67 | H67 | Wild type zebrafish control 2 | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | ILLUMINA | Illumina HiSeq 2500 | SRP159583 | H67_S2_L001_R2_001.fastq.gz H67_S2_L001_R1_001.fastq.gz | fastq fastq | 18477819300.0 | 61592731.0 | H67 S2 L001 R2 001.fastq.gz | 0:150 1:150 | A:4887782016;C:4375793599;G:4438933003;T:4775205744;N:104938 | 150 | 150 | 4887782016 | 4375793599 | 4438933003 | 4775205744 | 104938 | SRX4640375 | SRS3738589 | SRA766519 | Department of Haematology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University|Department: Department of Haematology | Department of Haematology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University | 2 | 0.93322 | 0.93319 | 0.06946 | 0.06963 | 0.6747 | 0.68083 | 0.47183 | 0.47148 | 150 | 150 | B | B | biological fallback assumption | illumina | hiseq_era | full_length | random_priming | unknown | bulk | unknown | unknown | China | 2019-09-20 | Adult | Adult | Blood | Hematopoietic System |