run_metadata: 48909
This data as json
| rowid | run.accession | experiment.accession | sample.accession | study.accession | bioproject | study.title | study.alias | study.type | study.abstract | study.attributes | study.PMIDs | sample.description | sample.title | sample.alias | sample.centername | sample.attributes | GEOsample.title | GEOsample.dataprocessing | GEOsample.source | GEOsample.treatmentprotocol | GEOsample.extractprotocol | GEOsample.growthprotocol | GEOsample.characteristics | GEOsample.accession | experiment.title | experiment.alias | experiment.library_name | experiment.design_description | experiment.library_construction_protocol | experiment.attributes | experiment.library_strategy | experiment.library_source | experiment.library_selection | experiment.library_layout | experiment.platform | experiment.instrument_model | experiment.spot_descriptor | experiment.study_ref | run.title | run.attributes | run.filename | run.semantic_name | run.total_bases | run.total_spots | run.alias | run.read_lengths | run.base_counts | run.r1_length | run.r2_length | run.r3_length | run.r4_length | run.Acount | run.Ccount | run.Gcount | run.Tcount | run.Ncount | run.experiment | run.pool_member | submission.accession | submission.srasource | submission.bioprojectsource | seqdetective.n_mates | seqdetective.mapping_rate.mate1 | seqdetective.mapping_rate.mate2 | seqdetective.nofeature_rate.mate1 | seqdetective.nofeature_rate.mate2 | seqdetective.sparsity.mate1 | seqdetective.sparsity.mate2 | seqdetective.pos_strand_rate.mate1 | seqdetective.pos_strand_rate.mate2 | seqdetective.readlen.mate1 | seqdetective.readlen.mate2 | seqdetective.judgement.mate1 | seqdetective.judgement.mate2 | seqdetective.judgement.reason | platform_family | instrument_generation | read_bias | selection_class | prep_kit | sc_or_bulk | tech_class | technology | tech_variant | submission.bioprojectsource.country | earliest_date | devstage_curation | devstage_curation_coarse | tissue_curation | tissue_curation_coarse |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 48909 | SRR7615221 | SRX4479802 | SRS3604791 | SRP155604 | PRJNA479418 | Lariat intronic RNAs in the cytoplasm of vertebrate cells | PRJNA479418 | Other | Introns are non coding DNA sequences interspersed among the coding sequences of genes. Shortly post transcription the intronic sequences are spliced out of the primary RNA transcript as lariat RNAs circular molecules with a short tail. Most of these lariats are destroyed within minutes in the cell nucleus. We report here that many such intronic RNAs are in fact exported to the cytoplasm where they remain as stable circular molecules. These cytoplasmic introns are derived from hundreds of different genes of widely different functions. We find them in cells of human mouse chicken frog and zebrafish. The widespread occurrence of so many stable lariat RNAs in the cytoplasm suggests that they play some as yet unexpected role in cell metabolism. | zebrafish eggs plusRNaseR | strain:AB strain|dev stage:Germline|sex:female|tissue:Egg|treatment:n1|rna treatment:rRNA depletion and RNase R|BioSampleModel:Model organism or animal | RNAseq of Zebrafish egg plus RNaseR | Zf pR | Zf pR | rRNA depletion ribozero fellowed by TruSeq Stranded Total RNA illumina | RNA-Seq | TRANSCRIPTOMIC | RANDOM | SINGLE | ILLUMINA | Illumina HiSeq 2000 | SRP155604 | 150112_Zebrafish_egg_plusRNaseR_50bp.fastq | fastq | 1453087900.0 | 29061758.0 | 150112 Zebrafish egg plusRNaseR 50bp.fastq | 0:50 | A:316388483;C:426462939;G:363020331;T:347178488;N:37659 | 50 | 316388483 | 426462939 | 363020331 | 347178488 | 37659 | SRX4479802 | SRS3604791 | SRA746415 | Carnegie Institution for Science|Department of Embryology | Carnegie Institution for Science | 1 | 0.83263 | 0.31979 | 0.81592 | 0.51542 | 50 | B | usable mapping rate | illumina | hiseq_era | unknown | random_priming | ribozero | bulk | unknown | unknown | United States | 2018-07-28 | Undetermined | Undetermined | Undetermined | Undetermined |