run_metadata: 48332

This data as json

rowid	run.accession	experiment.accession	sample.accession	study.accession	bioproject	study.title	study.alias	study.type	study.abstract	study.attributes	study.PMIDs	sample.description	sample.title	sample.alias	sample.centername	sample.attributes	GEOsample.title	GEOsample.dataprocessing	GEOsample.source	GEOsample.treatmentprotocol	GEOsample.extractprotocol	GEOsample.growthprotocol	GEOsample.characteristics	GEOsample.accession	experiment.title	experiment.alias	experiment.library_name	experiment.design_description	experiment.library_construction_protocol	experiment.attributes	experiment.library_strategy	experiment.library_source	experiment.library_selection	experiment.library_layout	experiment.platform	experiment.instrument_model	experiment.spot_descriptor	experiment.study_ref	run.title	run.attributes	run.filename	run.semantic_name	run.total_bases	run.total_spots	run.alias	run.read_lengths	run.base_counts	run.r1_length	run.r2_length	run.r3_length	run.r4_length	run.Acount	run.Ccount	run.Gcount	run.Tcount	run.Ncount	run.experiment	run.pool_member	submission.accession	submission.srasource	submission.bioprojectsource	seqdetective.n_mates	seqdetective.mapping_rate.mate1	seqdetective.mapping_rate.mate2	seqdetective.nofeature_rate.mate1	seqdetective.nofeature_rate.mate2	seqdetective.sparsity.mate1	seqdetective.sparsity.mate2	seqdetective.pos_strand_rate.mate1	seqdetective.pos_strand_rate.mate2	seqdetective.readlen.mate1	seqdetective.readlen.mate2	seqdetective.judgement.mate1	seqdetective.judgement.mate2	seqdetective.judgement.reason	platform_family	instrument_generation	read_bias	selection_class	prep_kit	sc_or_bulk	tech_class	technology	tech_variant	submission.bioprojectsource.country	earliest_date	devstage_curation	devstage_curation_coarse	tissue_curation	tissue_curation_coarse
48332	SRR7244613	SRX4149437	SRS3363033	SRP149500	PRJNA473641	Danio rerio strain:Tg vas:EGFP; figla \| isolate:WT GFP S/W \| breed:F3 generation Transcriptome or Gene expression	PRJNA473641	Transcriptome Analysis	Explore the function of figla gene in zebrafish gonadal development				Control weak GFP	WT GFP W 2		strain:Not applicable\|isolate:figla +/ \|breed:Not applicable\|cultivar:Not applicable\|ecotype:Not applicable\|age:25 dpf\|dev stage:Fish fry\|sex:Unknown\|tissue:Gonads\|replicate:Biological replicate 2 for WT GFP W\|BioSampleModel:Model organism or animal									RNA Seq of Danio rerio: 25 dpf gonad	WT GFP W 2	WT GFP W 2	RNA sequencing libraries were generated by extracting 3 g RNA per sample RNA from 25 dpf Danio rerio gonadal tissues. mRNA was then purified from the total RNA and fragmentation was carried out at elevated temperatures. Subsequent cDNA synthesis and size selection steps were carried out to generate fragments of 150 200 bp. PCR purification and quantification was carried out followed by clustering of the libraries. The libraries were then sequenced to generate 150 bp reads.			RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 4000		SRP149500			WT-GFP-W-2_2.fq.gz WT-GFP-W-2_1.fq.gz	fastq fastq	4411639200.0	14705464.0	WT GFP W 2 2.fq.gz	0:150 1:150	A:1131025272;C:1072829344;G:1075973911;T:1131098315;N:712358	150	150			1131025272	1072829344	1075973911	1131098315	712358	SRX4149437	SRS3363033	SRA713582	University of Macau\|Faculty of Health Sciences	University of Macau	2	0.93551	0.93638	0.03626	0.03662	0.73866	0.7416	0.50337	0.50374	150	150	B	B	biological fallback assumption	illumina	hiseq_era	unknown	poly_a	unknown	bulk	unknown	unknown		China	2018-06-01	Larval	Larval	Gonad	Reproductive System