run_metadata: 48038

This data as json

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48038	SRR6921820	SRX3869072	SRS3112047	SRP136856	PRJNA448207	Transcriptomic profiles of zebrafish liver exposed to phenanthrene	PRJNA448207	Other	The present study aims to examine the toxicological effects of phenanthrene in the molecular mechanism in zebrafish. We performed RNA Seq analysis on zebrafish liver responding to phenanthrene exposure. Genes involved in phenanthrene exposure to zebrafish liver were identified from the transcriptional data. This study provides the completed evaluation of toxic effect of phenanthrene exposure to zebrafish by transcriptional approaches. Our findings could present foundation for further study on molecular mechanism responsible for zebrafish's responses to phenanthrene exposure.			Fish in trplicate were anaesthetized with MS 222 at the dose of 10 mg/L and livers were sampled and pooled		PHE3d.2		strain:not applicable\|isolate:not applicable\|breed:AB line\|cultivar:not applicable\|ecotype:not applicable\|age:3 month\|dev stage:Adult fish\|sex:pooled male and female\|tissue:liver\|biomaterial provider:China Zebrafish Resource Center\|birth location:Wuhan China\|breeding method:maintained in the semi static system under conditions of water quality and illumination as follows: temperature 2327 pH 78 dissolved oxygen concentration 5 mg L 1 total hardness 10250 mg CaCO3 per liter and light/dark period of 12:12 h.\|genotype:AB line\|health state:Healthy\|sample type:tissue sample\|treatment:Fish exposed to phenanthrene for xxx day at concentration of 300 g L 1\|replicate:biological replicate 2\|BioSampleModel:Model organism or animal									RNA Seq of Danio rerio: adult male and female exposed to phenanthrene for xxx day	PHE3d.2	PHE3d.2	Random PCR			RNA-Seq	TRANSCRIPTOMIC	RANDOM PCR	PAIRED	ILLUMINA	Illumina HiSeq 4000		SRP136856			PHE3d_2_1.fq.gz PHE3d_2_2.fq.gz	fastq fastq	8406789300.0	28022631.0	PHE3d 2 1.fq.gz	0:150 1:150	A:2187414004;C:2019038004;G:2013512837;T:2181333490;N:5490965	150	150			2187414004	2019038004	2013512837	2181333490	5490965	SRX3869072	SRS3112047	SRA678027	Pearl River Fisheries Research Institute, CAFS\|Fisheries environmental protection laboratory	Pearl River Fisheries Research Institute, CAFS	2	0.91151	0.91449	0.01671	0.017	0.75315	0.76203	0.46837	0.47419	150	150	B	B	biological fallback assumption	illumina	hiseq_era	unknown	random_priming	unknown	bulk	unknown	unknown		China	2018-03-31	Adult	Adult	Liver	Liver and Biliary System