run_metadata: 43827
This data as json
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 43827 | SRR6152624 | SRX3264618 | SRS2576843 | SRP119651 | PRJNA413770 | Cross generational effects of a marginal 1 C metabolite deficiency | PRJNA413770 | Other | The effect of a deficiency in one carbon 1 C metabolites in the adult generation on DNA methylation and gene expression in adult offspring livers was investigated through the use of high throughput RNA sequencing and reduced representation bisulfite sequencing.Individuals in the F0 generation were either fed a normal diet or one marginally deficient in 1 C metabolites folate vitamins B12 and B6 methionine and choline from larval stages and mated within each diet group. The resulting offspring were fed the control diet throughout their lives and Livers of adult offspring were harvested post 113 dpf and RNA and DNA extracted for RNA sequencing and RRBS. | F1 livers low 1C 2 | rna low 1C 2 | strain:AB strain|isolate:Male fish tank 8|age:113 dpf|dev stage:adult|sex:male|tissue:liver|biomaterial provider:NIFES Kaja Helvik Skjaerven|birth location:NIFES|breeding method:Ref: Skjaerven K. H. et al. Sci Rep 6 34535 doi:10.1038/srep34535 2016|collected by:Kaja Helvik Skjaerven and Anne Catrin Adam|collection date:2013 11 12|health state:Good|BioSampleModel:Model organism or animal | F1 livers low 1C 2 | rna low 1C 2 | rna low 1C 2 | Directional sequencing of mRNA fragments | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | Illumina HiSeq 2000 | SRP119651 | 1250104800.0 | 25002096.0 | RZY 320.fastq | 0:50 | A:298225313;C:301950518;G:305099451;T:344161142;N:668376 | 50 | 298225313 | 301950518 | 305099451 | 344161142 | 668376 | SRX3264618 | SRS2576843 | SRA617983 | Nord University|FBA | Nord University | 1 | 0.88702 | 0.09849 | 0.82548 | 0.57466 | 50 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | Norway | 2017-10-10 | Adult | Adult | Liver | Liver and Biliary System |