run_metadata: 43751
This data as json
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 43751 | SRR6047141 | SRX3194196 | SRS2520597 | SRP117794 | PRJNA407650 | Danio rerio Transcriptome or Gene expression | PRJNA407650 | Transcriptome Analysis | We performed RNA sequencing on four groups of zebrafish larvae: control TgMyc TgKras TgMyc&TgKras to analyze the expression of genes involved in the lipid associated pathways.The results revealed high dynamic alterations in almost all aspects of lipid metabolism among which the expressions of genes involved in TG/DG/GP transformation and FA desaturation/elongation displayed intensive changes in consistent with our observations in lipodomics profiling | Kras | strain:Tgfabp10a:TetON; TRE:eGFP krasv12|breed:breed with AB|cultivar:not applicable|ecotype:not applicable|age:6dpf|dev stage:larval|sex:not determined|tissue:whole larvae|treatment:E3 with Doxcyclin42ug/ml|BioSampleModel:Model organism or animal | RNA seq of zebrafish larvaeKras at 6dpf | Kras | Kras | In our project we sequence 4 samples use Illumina Hiseq platform and on average we generated about 6.56Gb bases from each sample. We also map clean reads to reference genome on average 71.49% reads are mapped | RNA-Seq | TRANSCRIPTOMIC | RANDOM | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP117794 | FCHCKWTBBXX-WHZEBleaEAADRAAPEI-21_L1_1.fq.gz FCHCKWTBBXX-WHZEBleaEAADRAAPEI-21_L1_2.fq.gz | fastq fastq | 6567871200.0 | 21892904.0 | FCHCKWTBBXX WHZEBleaEAADRAAPEI 21 L1 2.fq.gz | 0:150 1:150 | A:1728249052;C:1564137653;G:1559231198;T:1715565790;N:687507 | 150 | 150 | 1728249052 | 1564137653 | 1559231198 | 1715565790 | 687507 | SRX3194196 | SRS2520597 | SRA608833 | Fudan University|Department of Biochemistry and Molecular Biology, | Fudan University | 2 | 0.93087 | 0.92811 | 0.06342 | 0.06328 | 0.66941 | 0.67493 | 0.47581 | 0.47601 | 150 | 150 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2017-09-17 | Larval | Larval | Whole Organism | All anatomical structures |