run_metadata: 43660
This data as json
| rowid | run.accession | experiment.accession | sample.accession | study.accession | bioproject | study.title | study.alias | study.type | study.abstract | study.attributes | study.PMIDs | sample.description | sample.title | sample.alias | sample.centername | sample.attributes | GEOsample.title | GEOsample.dataprocessing | GEOsample.source | GEOsample.treatmentprotocol | GEOsample.extractprotocol | GEOsample.growthprotocol | GEOsample.characteristics | GEOsample.accession | experiment.title | experiment.alias | experiment.library_name | experiment.design_description | experiment.library_construction_protocol | experiment.attributes | experiment.library_strategy | experiment.library_source | experiment.library_selection | experiment.library_layout | experiment.platform | experiment.instrument_model | experiment.spot_descriptor | experiment.study_ref | run.title | run.attributes | run.filename | run.semantic_name | run.total_bases | run.total_spots | run.alias | run.read_lengths | run.base_counts | run.r1_length | run.r2_length | run.r3_length | run.r4_length | run.Acount | run.Ccount | run.Gcount | run.Tcount | run.Ncount | run.experiment | run.pool_member | submission.accession | submission.srasource | submission.bioprojectsource | seqdetective.n_mates | seqdetective.mapping_rate.mate1 | seqdetective.mapping_rate.mate2 | seqdetective.nofeature_rate.mate1 | seqdetective.nofeature_rate.mate2 | seqdetective.sparsity.mate1 | seqdetective.sparsity.mate2 | seqdetective.pos_strand_rate.mate1 | seqdetective.pos_strand_rate.mate2 | seqdetective.readlen.mate1 | seqdetective.readlen.mate2 | seqdetective.judgement.mate1 | seqdetective.judgement.mate2 | seqdetective.judgement.reason | platform_family | instrument_generation | read_bias | selection_class | prep_kit | sc_or_bulk | tech_class | technology | tech_variant | submission.bioprojectsource.country | earliest_date | devstage_curation | devstage_curation_coarse | tissue_curation | tissue_curation_coarse |
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| 43660 | SRR6003528 | SRX3158823 | SRS2490160 | SRP116760 | PRJNA401346 | Raw sequence reads of miR 462/miR 731 knockdwon zebrafish embryos | PRJNA401346 | Other | To elucidate the biological roles of miR 462 731 during embryonic development two morpholinos MOs targeting the mature sequence of miR 462 and miR 731 respectively were microinjected into zebrafish embryos. A total of 100 embryos of uninjected control injected with miR 462 MO or injected with miR 731 MO were collected at xxx hpf respectively for RNA Seq. | 100 embyos micorinjected with morphorlinos of miR 462 | Danio rerio embryos | 462MO 48hpf | strain:AB|dev stage:48hpf|sex:not applicable|tissue:embryos|biomaterial provider:Key Laboratory of Freshwater Animal Breeding College of Fishery Huazhong Agricultural University|collected by:Chun Xiao Huang|sample type:whole embryo|treatment:micorinjected with morphorlinos of miR 462|BioSampleModel:Model organism or animal | RNA Seq of zebrafish: 48hpf embryos | 462MO 48hpf | 462MO 48hpf | Sequencing libraries were generated using NEBNext? Ultra? RNA LibraryPrep Kit for Illumina? NEB USA following manufacturer¡¯s recommendations and indexcodes were added to attribute sequences to each sample. | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | PAIRED | ILLUMINA | Illumina HiSeq 2500 | SRP116760 | 3643659000.0 | 14574636.0 | M462MO48 1.fq.gz | 0:125 1:125 | A:929370426;C:890981335;G:907736720;T:914809265;N:761254 | 125 | 125 | 929370426 | 890981335 | 907736720 | 914809265 | 761254 | SRX3158823 | SRS2490160 | SRA605241 | Huazhong Agricultural University|College of Fisheries | Huazhong Agricultural University | 2 | 0.94745 | 0.94585 | 0.03062 | 0.0299 | 0.71005 | 0.7167 | 0.47212 | 0.46832 | 125 | 125 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | other | nebnext | bulk | unknown | unknown | China | 2017-09-05 | Hatching | Embryo | Embryo Imprecise | All anatomical structures |