run_metadata: 43464
This data as json
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| 43464 | SRR5947673 | SRX3106438 | SRS2441701 | SRP115809 | PRJNA399156 | Best practices for genome wide RNA structure analysis: combination of mutational profiles and drop off information | PRJNA399156 | Other | Genome wide RNA structure maps have recently become available through the coupling of in vivo chemical probing reagents with next generation sequencing. Initial analyses relied on the identification of truncated reverse transcription reads to identify the chemically modified nucleotides but recent studies have shown that mutational signatures can also be used. While these two methods have been employed interchangeably here we show that they actually provide complementary information. Consequently analyses using exclusively one of the two methodologies may disregard a significant portion of the structural information. We find that the identity and sequence environment of the modified nucleotide greatly affects the odds of introducing a mismatch or causing reverse transcriptase drop off. Finally we identify specific mismatch signatures generated by dimethyl sulfate probing that can be used to remove false positives typically produced in RNA structurome analyses and how these signatures vary depending on the reverse transcription enzyme used. | Structure seq WT 64c DMS TGIRT | sseq diff rt AG01159 | strain:TU/AB|age:2.0|dev stage:64c|sex:pooled male and female|tissue:embryo|treatment:DMS 2% in vivo|molecule:RNA|selection:pA|condition:DMS|replicate group:4|replicate:1|BioSampleModel:Model organism or animal | Structure seq WT 64c DMS TGIRT | AG01159.1 | AG01159.1 | RNA | OTHER | TRANSCRIPTOMIC | unspecified | SINGLE | ILLUMINA | Illumina HiSeq 2000 | SRP115809 | AG01159.1_R1.fastq.gz | fastq | 658716700.0 | 8667325.0 | AG01159.1 R1.fastq.gz | 0:76 | A:200810238;C:143643567;G:154463128;T:159778992;N:20775 | 76 | 200810238 | 143643567 | 154463128 | 159778992 | 20775 | SRX3106438 | SRS2441701 | SRA600818 | Yale_Giraldez|Genetics | Yale_Giraldez_Group | 1 | 0.81918 | 0.03506 | 0.76733 | 0.52575 | 76 | B | usable mapping rate | illumina | hiseq_era | unknown | unknown | unknown | bulk | unknown | unknown | United States | 2017-08-23 | Cleavage | Embryo | Embryo Imprecise | All anatomical structures |