run_metadata: 43
This data as json
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 43 | DRR408243 | DRX393849 | DRS407174 | DRP012042 | PRJDB14275 | Zebrafish EN/ENCDC RNA seq | DRP012042 | Transcriptome Analysis | A project to find differential expressed genes between enteric neurons ENs and enteric neural crest derived cells ENCDCs in larval zebrafish gut. We dissected guts of transgenic line TgSAGFFLF217B; uas:gfp for ENs and Tgsox10:cre; EF1alpha:loxP gfp loxP dsred for ENCDCs and isolated GFP+ ENs and dsRed+ ENCDCs. Three duplicates for each of ENs and ENCDCs are prepared. Libraries for NGS are prepared using SMART Seq V4 Ultra Low Input RNA Kit. | zebrafish 5 day GFP positive enteric neurons replicate 1 | zebrafish EN replicate 1 | SAMD00529463 | sample name:zebrafish EN replicate 1|biological replicate:eneteric neurons 1|strain:TgSAGFFLF217B; uas:gfp | NextSeq 550 paired end sequencing of SAMD00529463 | DRX393849 | 190326ENvsNC N701 5day;EntericNeuron;rep1 | 1 | 1 | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | NextSeq 550 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>160</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>81</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | DRP012042 | NextSeq 550 paired end sequencing of SAMD00529463 | 2999501518.0 | 19455440.0 | DRR408243 | 0:77.08 1:77.09 | A:788053541;C:705895776;G:724185148;T:775760738;N:5606315 | 77 | 77 | 788053541 | 705895776 | 724185148 | 775760738 | 5606315 | DRX393849 | DRS407174 | DRA014886 | NIBB|NIBB core research facilities, National Institute for Basic Biology | University of Hyogo | B | B | biological fallback assumption | illumina | nextseq | unknown | random_priming | unknown | bulk | unknown | unknown | Japan | 2024-09-22 | Undetermined | Larval | Undetermined | Undetermined |