run_metadata: 42238
This data as json
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 42238 | SRR5837509 | SRX3013981 | SRS2363577 | SRP112722 | PRJNA385064 | Transcriptomic effects of 17 alpha methyltestosterone on zebrafish brain sexual development. | PRJNA385064 | Other | Sexual differentiation in zebrafish is complex. Although zebrafish sex determination is primarily genetic hormonal and environmental factors can influence sexual development. Androgen treatment triggers phenotypic and behavioural masculinisation in zebrafish. 17 alpha methyltestosterone MT a synthetic androgen is routinely used in aquaculture for production of all male populations. However the molecular mechanisms underlying 17 alpha methyltestosterone induced brain and behavioural masculinisation in fish are poorly understood. In this study we analysed brain transcriptomes of zebrafish treated with 17 alpha methyltestosterone during gonadal development from 20 dpf to 40 dpf and 60 dpf and compared them with brain transcriptomes of untreated female and male zebrafish. These data give insight into the role of androgens in teleost brain sexual differentiation. | 60CMB1 | strain:Tgvas:egfp|age:60 dpf|dev stage:juvenile|sex:male|tissue:brain|genotype:Tgvas:egfp|sample type:whole brain|treatment:untreated|replicate:60 dpf control male brain Replicate 1|BioSampleModel:Model organism or animal | RNA Seq of danio rerio: 60 dpf untreated male brain | 60CMB1 | 60CMB1 | 1 µg of total RNA was used with the Illumina TruSeq RNA sample preparation kit v2 for construction of each library. 100 bp paired end reads were generated using the HiSeq 2000 platform. | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | ILLUMINA | Illumina HiSeq 2000 | SRP112722 | BC1JJDACXX_NZGL00075_60SCMB1_CGATGT_L005_R1_001.fastq.gz BC1JJDACXX_NZGL00075_60SCMB1_CGATGT_L005_R2_001.fastq.gz C1PF2ACXX_NZGL00075_60SCMB1_CGATGT_L003_R1_001.fastq.gz C1PF2ACXX_NZGL00075_60SCMB1_CGATGT_L003_R2_001.fastq.gz C1PF2ACXX_NZGL00075_60SCMB1_CGATGT_L004_R1_001.fastq.gz C1PF2ACXX_NZGL00075_60SCMB1_CGATGT_L004_R2_001.fastq.gz | fastq fastq fastq fastq fastq fastq | 3656502200.0 | 18282511.0 | C1PF2ACXX NZGL00075 60SCMB1 CGATGT L004 R1 001.fastq.gz | 0:100 1:100 | A:1006097362;C:825773612;G:813254919;T:1003161970;N:8214337 | 100 | 100 | 1006097362 | 825773612 | 813254919 | 1003161970 | 8214337 | SRX3013981 | SRS2363577 | SRA588281 | University of Otago|Anatomy | University of Otago | 2 | 0.9381 | 0.93994 | 0.11219 | 0.113 | 0.69002 | 0.69053 | 0.49163 | 0.48747 | 100 | 100 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | cdna_unspecified | trueseq | bulk | unknown | unknown | New Zealand | 2018-07-31 | Juvenile | Juvenile | Brain | Nervous System |