run_metadata: 39920
This data as json
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 39920 | SRR2353207 | SRX1225279 | SRS1065174 | SRP063624 | PRJNA295427 | Expression profiling of centroacinar cells from adult zebrafish pancreas | GSE72963 | Transcriptome Analysis | We sequenced mRNA from two preparations of isolated Notch responsive ductal pancreas cells and compared transcript expression to all other non Notch responsive cells from each sample to charactarize zebrafish centroacinar cells. Overall design: Determination of gene expression levels in centroacinar cells and non centroacinar cells from adult pancreas. | pubmed:26153247 | CAC rep 1 | GSM1875472 | source name:pancreas CAC|tissue:pancreas|developmental stage:adult|strain:AB|cell type:isolated centroacinar cells | CAC rep 1 | Reads were processed and mapped to Zv9/danRer7 using RSEM EBseq was used to determine differential expression and significance values Genome build: Zv9/danRer7 Supplementary files format and content: tab delimited text files using corrected and uncorrected foldchange values | pancreas CAC | Pancreas was dissected on ice and dissociated. CACs and non CACs were sorted by FACS and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit Cat#FC 122 1001 was used for the construction of sequencing libraries. Libraries were constructed using standard Illumina protocols | tissue:pancreas|developmental stage:adult|strain:AB|cell type:isolated centroacinar cells | GSM1875472 | GSM1875472: CAC rep 1; Danio rerio; RNA Seq | GSM1875472 | 1 | Pancreas was dissected on ice and dissociated. CACs and non CACs were sorted by FACS and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit Cat#FC 122 1001 was used for the construction of sequencing libraries. Libraries were constructed using standard Illumina protocols | GEO Accession:GSM1875472 | RNA-Seq | TRANSCRIPTOMIC | cDNA | SINGLE | ILLUMINA | Illumina Genome Analyzer II | SRP063624 | H0J49ADXXs_1_1_merged.fastq.bz2 | fastq | 8167909125.0 | 108905455.0 | GSM1875472 r1 | 0:75 | A:2418955841;C:1674627530;G:1764871153;T:2308386909;N:1067692 | 75 | 2418955841 | 1674627530 | 1764871153 | 2308386909 | 1067692 | SRX1225279 | SRS1065174 | SRA297337 | GEO | Institute of Genetic Medicine, Johns Hopkins University | 1 | 0.66215 | 0.21771 | 0.72437 | 0.57789 | 75 | B | usable mapping rate | illumina | early_illumina | unknown | cdna_unspecified | trueseq | bulk | unknown | unknown | United States | 2015-09-11 | Adult | Adult | Pancreas | Endocrine System |