run_metadata: 37274
This data as json
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| 37274 | SRR1050716 | SRX392660 | SRS515659 | SRP034513 | PRJNA231764 | Transcriptomic analyses of Myc and xmrk induced zebrafish liver cancer | GSE53342 | Transcriptome Analysis | To compare the characteristics and mechanisms of Myc and xmrk induced zebrafish liver tumor next generation sequencing based SAGE analyses were used to examine the transcriptomes of tumor and control samples. The results indicated that relatively small overlaps of significantly deregulated genes and biological pathways among different zebrafish liver tumor models.Nevertheless they all significantly correlate with advanced or very advanced human hepatocellular carcinoma HCC. Molecular signature from each oncogene induced zebrafish liver tumor correlated with only a small subset of human HCC samples and they share conserved up regulated pathways. A short list of commonly deregulated genes among different zebrafish liver tumors showed accordant deregulation in the majority of human HCCs suggesting that they may serve as common diagnosis markers and therapeutic targets.Thus these transgenic zebrafish models with well defined oncogene induced tumors are valuable tools for molecular classification of human HCCs and for understanding of molecular drivers in hepatocarcinogenesis in each human HCC subgroup. Overall design: Transcriptome profiling of Myc tumor sample X M+D+ and control samples X M D X M+D X M D+ xmrk tumor sample X+M D+ and control samples X M D X+M D X M D+ were generated by deep sequencing using three prime RNA SAGE on SOLiD system | pubmed:24633177 | X+M D | GSM1289480 | source name:control liver|tissue:liver|genotype:Tgfabp10:TA; TRE:xmrk; krt4:GFP|agent:n1 | X+M D | The SOLiD generated RNA Seq reads was in 27 bp length and an initial filtering process was taken to remove any non desirable contamination sequences. The reads were mapped to the zebrafish RefSeq mRNA database allowing maxium mismatches. The expression levels of mapped transcripts were normalized into transcript per million TPM to faciliate comparison among different samples. Genome build: danRer7 Supplementary files format and content: The mapped transcripts were listed with GI and RefSeq ID together with expression levels in TPM. | control liver | Transgenic zebrafish and their wildtype siblings were treated starting from 3.5 mpf. Doxycycline treatment was conducted in 6 L tanks with around 25 adult and water was changed every other day. | Total RNA was extracted using TRIzol Reagent Invitrogen USA and treated with DNase I to remove genomic DNA contamination. Library construction was conducted by Mission Biotech Taiwan following standard ABI SOLiD protocol. | tissue:liver|genotype:Tgfabp10:TA; TRE:xmrk; krt4:GFP|agent:n1 | GSM1289480 | GSM1289480: X+M D ; Danio rerio; RNA Seq | GSM1289480 | 1 | Total RNA was extracted using TRIzol Reagent Invitrogen USA and treated with DNase I to remove genomic DNA contamination. Library construction was conducted by Mission Biotech Taiwan following standard ABI SOLiD protocol. | GEO Accession:GSM1289480 | RNA-Seq | TRANSCRIPTOMIC | cDNA | SINGLE | ABI_SOLID | AB SOLiD System 3.0 | SRP034513 | X+M-D-.csfasta.gz X+M-D-.qual | SOLiD_native SOLiD_native | 634523960.0 | 18129256.0 | GSM1289480 r1 | 0:35 | 0:234010528;1:135057441;2:132344184;3:131577105;.:1534702 | 35 | SRX392660 | SRS515659 | SRA117588 | GEO | National University of Singapore | 1 | 0.06814 | 0.05142 | 0.99579 | 0.6174 | 35 | B | usable mapping rate | legacy | early | unknown | cdna_unspecified | unknown | bulk | unknown | unknown | Singapore | 2013-12-16 | Adult | Adult | Liver | Liver and Biliary System |