run_metadata: 36212
This data as json
| rowid | run.accession | experiment.accession | sample.accession | study.accession | bioproject | study.title | study.alias | study.type | study.abstract | study.attributes | study.PMIDs | sample.description | sample.title | sample.alias | sample.centername | sample.attributes | GEOsample.title | GEOsample.dataprocessing | GEOsample.source | GEOsample.treatmentprotocol | GEOsample.extractprotocol | GEOsample.growthprotocol | GEOsample.characteristics | GEOsample.accession | experiment.title | experiment.alias | experiment.library_name | experiment.design_description | experiment.library_construction_protocol | experiment.attributes | experiment.library_strategy | experiment.library_source | experiment.library_selection | experiment.library_layout | experiment.platform | experiment.instrument_model | experiment.spot_descriptor | experiment.study_ref | run.title | run.attributes | run.filename | run.semantic_name | run.total_bases | run.total_spots | run.alias | run.read_lengths | run.base_counts | run.r1_length | run.r2_length | run.r3_length | run.r4_length | run.Acount | run.Ccount | run.Gcount | run.Tcount | run.Ncount | run.experiment | run.pool_member | submission.accession | submission.srasource | submission.bioprojectsource | seqdetective.n_mates | seqdetective.mapping_rate.mate1 | seqdetective.mapping_rate.mate2 | seqdetective.nofeature_rate.mate1 | seqdetective.nofeature_rate.mate2 | seqdetective.sparsity.mate1 | seqdetective.sparsity.mate2 | seqdetective.pos_strand_rate.mate1 | seqdetective.pos_strand_rate.mate2 | seqdetective.readlen.mate1 | seqdetective.readlen.mate2 | seqdetective.judgement.mate1 | seqdetective.judgement.mate2 | seqdetective.judgement.reason | platform_family | instrument_generation | read_bias | selection_class | prep_kit | sc_or_bulk | tech_class | technology | tech_variant | submission.bioprojectsource.country | earliest_date | devstage_curation | devstage_curation_coarse | tissue_curation | tissue_curation_coarse |
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| 36212 | SRR33570992 | SRX28800977 | SRS25038275 | SRP585332 | PRJNA1262902 | BCAS2 promotes primitive hematopoiesis by sequestering ß catenin within the nucleus | GSE297155 | Transcriptome Analysis | Breast carcinoma amplified sequence 2 BCAS2 a core component of the hPrP19 complex plays crucial roles in various physiological and pathological processes. However whether BCAS2 has functions other than being a key RNA splicing regulator within the nucleus remains unknown. Here we show that BCAS2 is essential for primitive hematopoiesis in zebrafish and mouse embryos. The activation of Wnt/ß catenin signal which is required for hematopoietic progenitor differentiation is significantly decreased upon depletion of bcas2 in zebrafish embryos and mouse embryonic fibroblasts MEFs. Interestingly BCAS2 de?ciency has no obvious impact on the splicing efficiency of ß catenin pre mRNA while significantly attenuating ß catenin nuclear accumulation. Moreover we find that BCAS2 directly binds to ß catenin via its coiled coil domains thereby sequestering ß catenin within the nucleus. Thus our results uncover a previously unknown function of BCAS2 in promoting Wnt signaling by enhancing ß catenin nuclear retention during primitive hematopoiesis. Overall design: RNA sequencing profiling for wild type and bcas2 +/?14 embryos at 10 ss. | b14 | GSM8984757 | source name:embryo|tissue:embryo|genotype:heterozygous|geo loc name:missing|collection date:missing | b14 | The difference in the number of alternative splicing events between groups was analyzed using rMATS version 4.1.0 Assembly: GRCz11 Supplementary files format and content: Gene expression matrix | embryo | Embryos were collected at the 10 somite stage and gently transferred into lysis buffer. Reverse transcription was performed using a SMARTer Ultra Low RNA Kit Clontech 634437 directly from the cell lysates. The cDNA library was prepared using an Advantage 2 PCR Kit Clontech，639206 and then sequenced via the Illumina sequencing platform NovaSeq 6000. | tissue:embryo|genotype:heterozygous | GSM8984757 | GSM8984757: b14; Danio rerio; RNA Seq | GSM8984757 r1 | GSM8984757 | 1 | Embryos were collected at the 10 somite stage and gently transferred into lysis buffer. Reverse transcription was performed using a SMARTer Ultra Low RNA Kit Clontech 634437 directly from the cell lysates. The cDNA library was prepared using an Advantage 2 PCR Kit Clontech，639206 and then sequenced via the Illumina sequencing platform NovaSeq 6000. | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP585332 | b14_1.fq.gz b14_2.fq.gz | fastq fastq | 19276565400.0 | 64255218.0 | GSM8984757 r1 | 0:150 1:150 | A:5518694219;C:3928662779;G:4574767411;T:5253904649;N:536342 | 150 | 150 | 5518694219 | 3928662779 | 4574767411 | 5253904649 | 536342 | SRX28800977 | SRS25038275 | SRA2129895 | Affiliated Hospital of Guangdong Medical University | Affiliated Hospital of Guangdong Medical University | B | B | biological fallback assumption | illumina | novaseq_era | full_length | cdna_unspecified | smarter | bulk | unknown | unknown | China | 2025-05-14 | Segmentation | Embryo | Embryo Imprecise | All anatomical structures |