run_metadata: 34493
This data as json
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| 34493 | SRR31852179 | SRX27211967 | SRS23661290 | SRP554424 | PRJNA1204310 | Trained immunity amplifies intestinal complement 3 to maintain Th17 cells against bacterial infection induced enteritis in zebrafish | GSE285528 | Transcriptome Analysis | Infectious enteritis is often accompanied with immuno disorder of intestinal immune cells caused by microbials infection. Trained immunity is classically characterized by long term functional reprogramming of innate immune cells to combat infectious diseases. However whether the induction of trained immunity plays a role in protecting infectious enteritis remains largely unknown. Here through establishing an in vivo ß glucan training and E. piscicida infection model in zebrafish we observe that induction of trained immunity could alleviate bacterial infection caused enteritis. Moreover we identify intestinal complement C3 as a crucial target of trained immunity and could be amplified in response to bacterial infection. Furthermore we reveal that trained immunity could reverse the reduction of intestinal Th17 cells in C3 dependent manner to alleviate infectious enteritis. Taken together our results uncover the role of complement C3 mediated trained immunity in maintaining Th17 cells and intestine homeostasis and provide a theoretical strategy for immunotherapies of infectious enteritis. Overall design: RNA seq profiling of wildtype zebrafish in mock and PBS or ß glucan trained at Day 7 post secondary E. piscicida infection | Zebrafish BG rep1 | GSM8703889 | source name:Intestine|tissue:Intestine|strain:AB|genotype:WT|treatment:Beta Glucan training and E. piscicida infection|time:Day 7|geo loc name:missing|collection date:missing | Zebrafish BG rep1 | Constructing an index of the reference genome using HISAT2 v2.1.0 Assembly: GRCz21 Supplementary files format and content: tab delimited text file includes raw counts for each Sample Supplementary files format and content: csv files include FPKM values for each Sample | Intestine | 10 µL yeast derived β glucan 10 mg/mL was intraperitoneally injected i.p. and post resting for 5 days 5 µL E. piscicida2 × 106 CFU/mL was challenged with through rectal injection | The RNA of each individual sample was isolated by Trizol Invitrogen and chloroform. RNA libraries for RNA seq were prepared using NEBNext Ultra II RNA Library Prep Kit for Illumina following manufacturer's protocols. | Three to six mpf AB line zebrafish were maintained at 27 ± 1 °C and under a controlled light/dark cycle 14 h light/10 h dark. | tissue:Intestine|strain:AB|genotype:WT|treatment:Beta Glucan training and E. piscicida infection|time:Day 7 | GSM8703889 | GSM8703889: Zebrafish BG rep1; Danio rerio; RNA Seq | GSM8703889 r1 | GSM8703889 | 1 | The RNA of each individual sample was isolated by Trizol Invitrogen and chloroform. RNA libraries for RNA seq were prepared using NEBNext Ultra II RNA Library Prep Kit for Illumina following manufacturer's protocols. | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | ILLUMINA | Illumina HiSeq 1500 | SRP554424 | BG_1_R1.fastq.gz BG_1_R2.fastq.gz | fastq fastq | 6720003132.0 | 22251666.0 | GSM8703889 r1 | 0:151 1:151 | A:1771169227;C:1572565291;G:1597071360;T:1779087569;N:109685 | 151 | 151 | 1771169227 | 1572565291 | 1597071360 | 1779087569 | 109685 | SRX27211967 | SRS23661290 | SRA2042566 | Ahua lab, East China University of Science and Technology | Ahua lab, East China University of Science and Technology | B | B | biological fallback assumption | illumina | hiseq_era | unknown | cdna_unspecified | nebnext | bulk | unknown | unknown | China | 2024-12-30 | Adult | Adult | Gut | Digestive System |