run_metadata: 33985
This data as json
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| 33985 | SRR31022896 | SRX26409987 | SRS22929899 | SRP539058 | PRJNA1173962 | Study on the changes of gene transcription levels in zebrafish larvae treated to prothiobacillazole solution | PRJNA1173962 | Other | ProthiobacillazolePTA is a kind of widely used bactericidal pesticide. Due to its high water solubility and more negative reports on aquatic organisms in recent yrs it is necessary to further study its environment.Zebrafish as an in vivo research model has its unique advantages in evaluating drug toxicity and safety as it can efficiently evaluate multiple organ toxicities at the same time. In this study the PTA exposed and Control zebrafish larvae were collected at xxxdpf for each group of 4 replicates.The sample is used for RNA seq. | Repeat one:the PTA solution was exposed and cultured ttwo xxxdpf | PTA | PTA1 | strain:not applicable|isolate:missing|breed:missing|cultivar:not applicable|ecotype:missing|age:4dpf stage:larval phase|collection date:2024 04|geo loc name:missing|sex:missing|tissue:missing|biomaterial provider:Fish stress response and immune response Research team|birth date:missing|birth location:missing|breeding history:missing|breeding method:missing|cell line:not applicable|cell subtype:missing|cell type:missing|collected by:ChenGuo|culture collection:missing|death date:missing|disease:missing|disease stg:missing|genotype:missing|growth protocol:missing|health state:health|isolation source:missing|lat lon:missing|phenotype:missing|sample type:missing|specimen voucher:missing|store cond:missing|stud book number:missing|treatment:missing|costumer description:5|BioSampleModel:Model organism or animal | RNA seq of zebrafish | PTA1 | PTA1 | Use the Invitrogen TRIzol kit to extract total RNA. Select total RNA with a total amount of at least 1ug and use the NEBNext Ultra II RNA Library Prep Kit for Illumina New England Biolabs Inc; Ipswich Massachusetts USA to construct sequencing libraries. | RNA-Seq | TRANSCRIPTOMIC | cDNA | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP539058 | PTA1_R1.fastq.gz PTA1_R2.fastq.gz | fastq fastq | 7610150246.0 | 25199173.0 | PTA1 R1.fastq.gz | 0:151 1:151 | A:2078407024;C:1716455571;G:1761054093;T:2053740332;N:493226 | 151 | 151 | 2078407024 | 1716455571 | 1761054093 | 2053740332 | 493226 | SRX26409987 | SRS22929899 | SRA1992822 | Nanchang University|College of Life Science | Nanchang University | B | B | biological fallback assumption | illumina | novaseq_era | unknown | cdna_unspecified | nebnext | bulk | unknown | unknown | China | 2024-10-17 | Larval | Larval | Cell Line | Cell Line |